DYNAMICS OF EPITHELIAL-CELLS IN THE CORPUS OF THE MOUSE STOMACH .3. INWARD MIGRATION OF NECK CELLS FOLLOWED BY PROGRESSIVE TRANSFORMATION INTO ZYMOGENIC CELLS
DYNAMICS OF EPITHELIAL-CELLS IN THE CORPUS OF THE MOUSE STOMACH .3. INWARD MIGRATION OF NECK CELLS FOLLOWED BY PROGRESSIVE TRANSFORMATION INTO ZYMOGENIC CELLS
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DOI：
10.1002/ar.1092360204
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发表时间：
1993-06-01
影响因子：
--
通讯作者：
LEBLOND, CP
中科院分区：
文献类型：
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作者：
KARAM, SM;LEBLOND, CP
通过单次或多次注射或连续输注H-3-胸苷1-52天后，在电子显微镜（EM）和放射自显影中检查了小鼠体中颈部区域的颈细胞（或粘液颈细胞）和单位基部区域的酶原细胞（或主细胞）。对于这些研究，单元的颈部区域被细分为三个相等的部分，分别称为高颈、中颈和低颈，而基部区域被类似地细分为高基部、中基部和低基部。颈区平均包括12.6个颈细胞，其特征在于在EM中由深色粘液分泌颗粒组成，这些颗粒经常表现出浅色胃蛋白酶原核心。连续H-3-胸苷输注揭示，颈细胞来自颈前细胞，这被认为是出现在峡部区域从未分化的无颗粒细胞通过颈前细胞前体阶段。前颈细胞的特征是存在一些核心分泌颗粒，向内迁移（即，在单元的盲端的方向上）并进入颈部区域以成为颈部单元。据估计，59%的颈细胞来自颈前细胞的分化，而其他41%来自它们自己的有丝分裂。颈细胞在1-2周内从颈高段向颈中段和颈低段向内迁移，同时它们继续产生更多和更大的分泌颗粒，从而进一步分化为粘液产生细胞。当颈细胞到达高基节时，它们成为前发酵细胞，产生分泌颗粒，其中出现浅色、不规则的胃蛋白酶原斑块，这些斑块侵入深色粘液内容物。随着时间的推移，平均每单位有5.0个酶原细胞的前酶原细胞不断产生新的颗粒，这些颗粒具有越来越大的光斑，因此最终细胞产生的颗粒完全被光的胃蛋白酶原材料填充。在这个阶段，细胞是产酶细胞。平均每单位67.5个的产酶细胞进一步向内迁移，同时逐渐扩大并产生大小增加的胃蛋白酶原颗粒。在低基节中，一些酶原细胞显示变性迹象，导致坏死或凋亡死亡。虽然坏死细胞的残余物似乎被释放到单位腔，但凋亡细胞被邻近的酶原细胞或结缔组织巨噬细胞吞噬，从而突破泌酸单位的基底膜。简单地说，酶原细胞谱系是从静止的未分化的无颗粒细胞起始的细胞序列，包括前颈细胞前体、前颈细胞、颈细胞、前酶原细胞和最后的酶原细胞，它们都向单位的盲端方向迁移，在盲端附近酶原细胞丢失。
The neck cells (or mucous neck cells) present in the neck region and the zymogenic cells (or chief cells) present in the base region of the units in the mouse corpus were examined in the electron microscope (EM) and in radioautographs prepared after administration of H-3-thymidine by single or multiple injections or by continuous infusion for 1-52 days. For these studies, the neck region of the units has been subdivided into three equal segments, respectively named high neck, mid neck, and low neck, while the base region has been similarly subdivided into high base, mid base, and low base. The neck region includes an average of 12.6 neck cells, characterized in the EM by dark, mucous secretory granules that frequently exhibit a light, pepsinogenic core. Continuous H-3-thymidine infusion reveals that neck cells come from pre-neck cells, which are believed to arise in the isthmus region from the undifferentiated granule-free cells through a pre-neck cell precursor stage. The pre-neck cells, characterized by the presence of a few cored secretory granules, migrate inward (i.e., in the direction of the blind end of the units) and enter the neck region to become neck cells. It is estimated that 59% of the neck cells arise from differentiation of pre-neck cells, whereas the other 41% are derived from their own mitoses. Neck cells migrate inward in 1-2 weeks from the high through the mid and low neck segments, while they keep on producing more and larger secretory granules and thus further differentiate as mucus-producing cells. When neck cells reach the high base segment, they become pre-zymogenic cells that produce secretory granules in which appear light, irregular, pepsinogenic patches which encroach on the dark mucous content. With time, the pre-zymogenic cells, of which there are 5.0 per unit on the average, keep on producing new granules with larger and larger light patches, so that in the end the cells produce granules which are entirely filled by light, pepsinogenic material. At this stage, the cells are zymogenic cells. Zymogenic cells, which average 67.5 per unit, further migrate inward, while gradually enlarging and producing pepsinogenic granules of increasing size. In the low base segment, some zymogenic cells show signs of degeneration leading to death by either necrosis or apoptosis. While remnants of the necrotic cells appear to be released to the unit lumen, the apoptotic cells are phagocytosed by a neighboring zymogenic cell or by a connective tissue macrophage breaking through the basement membrane of the oxyntic unit. Briefly, the zymogenic cell lineage, a cell sequence initiated from the stationary undifferentiated granule-free cells, includes pre-neck cell precursors, pre-neck cells, neck cells, pre-zymogenic cells, and finally zymogenic cells, which all migrate in the direction of the unit's blind end, near which zymogenic cells are lost.