Influence of feed components on symbiotic bacterial community structure in the gut of the wood-feeding higher termite Nasutitermes takasagoensis
Influence of feed components on symbiotic bacterial community structure in the gut of the wood-feeding higher termite Nasutitermes takasagoensis
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DOI：
10.1271/bbb.60672
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发表时间：
2007-05-01
影响因子：
1.6
通讯作者：
Tanaka, Hideo
中科院分区：
文献类型：
--
作者：
Miyata, Ryo;Noda, Naohiro;Tanaka, Hideo
研究了不同碳源对食木高等白蚁（nasuttermes takasagoensis）肠道细菌群落结构的影响。16S rRNA基因测序和末端限制性片段长度多态性（T-RFLP）分析显示，在门水平上，细菌群落结构随饲料成分的不同而发生显著变化。木食和木粉食白蚁的无性系文库中以螺旋体纲为主，木聚糖、纤维素糖和葡萄糖食白蚁的无性系文库中以拟杆菌门为主，而木糖食白蚁的无性系文库中以厚壁菌门为主。此外，在木糖食用型白蚁文库中还发现了白蚁I群（TG1）门的无性系。我们的研究结果表明，白蚁与肠道微生物的共生关系在短期内不是很强或稳定的，白蚁肠道微生物群落结构随饲料成分的不同而变化。
The influence of carbon sources on bacterial community structure in the gut of the wood-feeding higher termite Nasutitermes takasagoensis was investigated. 16S rRNA gene sequencing and terminal-restriction fragment length polymorphism (T-RFLP) analyses revealed that the bacterial community structure changed markedly depending on feed components at the phylum level. Spirochaetes was predominant in the clone libraries from wood- and wood powder-fed termites, whereas Bacteroidetes was the largest group in the libraries from xylan-, cellobiose-, and glucose-fed termites, and Firmicutes was predominant in the library from xylose-fed termites. In addition, clones belonging to the phylum Termite Group I (TG1) were found in the library from xylose-fed termites. Our results indicate that the symbiotic relationship between termite and gut microorganisms is not very strong or stable over a short time, and that termite gut microbial community structures vary depending on components of the feeds.