AN AUTORADIOGRAPHIC STUDY OF THE RELATION BETWEEN HEMOCYTES AND CONNECTIVE TISSUE IN THE WAX MOTH, GALLERIA MELLONELLA L
AN AUTORADIOGRAPHIC STUDY OF THE RELATION BETWEEN HEMOCYTES AND CONNECTIVE TISSUE IN THE WAX MOTH, GALLERIA MELLONELLA L
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蜡蛾血细胞与结缔组织关系的放射自显影研究，Galleria Mellonella L
DOI：
10.2307/1539560
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发表时间：
1965
期刊：
影响因子：
--
通讯作者：
A. Richards
中科院分区：
文献类型：
--
作者：
S. C. Shrivastava;A. Richards
1. 大蜡螟在预蛹和早期蛹阶段的血细胞计数随着年龄的变化从 4000/mm.3 到 15000/m3 变化。通过对昆虫放血，自由循环细胞的数量可在 6-24 小时内大约增加一倍。2．利用氚化胸苷和放射自显影发现，存在原血细胞→浆细胞→脂肪血细胞的血细胞分化顺序。这种分化需要在35°下大约三天，再过三天标记的细胞就消失了。这些细胞的预期寿命，至少在蛹前和蛹早期，不到六天。3．输入标记的血细胞，然后进行切片放射自显影，证实了先前的建议，即脂肪血细胞在幼虫神经层的破坏中具有一定作用，但没有血细胞参与成虫神经系统周围结缔组织的形成。
1. The hemocyte count of Galleria mellonella during the prepupal and early pupal stages varies from 4000/mm.3 to 15000/m3 as a function of age. The number of freely circulating cells can be approximately doubled within 6-24 hours by bleeding the insect.2. Using tritiated thymidine and autoradiography it was found that there is a hemocyte differentiation sequence of prohemocyte → plasmatocyte → adipohemocyte. This differentiation requires about three days at 35° , and the labeled cells have disappeared in another three days. The life expectancy of these cells, at least in prepupal and early pupal stages, is then less than six days.3. Transfusion of labeled hemocytes, followed by autoradiography of sections, confirmed the previous suggestion that adipohemocytes have some role in the destruction of the larval neural lamella, but that no blood cells are involved in the formation of the connective tissue around the adult nervous system.