Activity of Insula to Basolateral Amygdala Projecting Neurons is Necessary and Sufficient for Taste Valence Representation
Activity of Insula to Basolateral Amygdala Projecting Neurons is Necessary and Sufficient for Taste Valence Representation
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DOI：
10.1523/jneurosci.0752-19.2019
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发表时间：
2019-11-20
影响因子：
5.3
通讯作者：
Rosenblum, Kobi
中科院分区：
文献类型：
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作者：
Kayyal, Haneen;Yiannakas, Adonis;Rosenblum, Kobi
条件性味觉厌恶（CTA）是一种联想学习范式，其中，食用一种开胃剂（如糖精）与服用一种诱发不适的药物（如腹腔注射LiCl）配对。厌恶味觉的学习和恢复需要脑岛前部（aIC）和杏仁核基底外侧（BLA）内的神经元活动。在这里，我们用逆转录aav结构标记了成年雄性小鼠的aIC投射到BLA的神经元，并评估了它们在厌恶和食欲味觉学习中的必要性。通过限制aICto-BLA神经元中化学发生受体的表达，我们证明aICto-BLA投射内的活动对于厌恶味觉记忆的获得和检索是必要的，但对于其维持和消除却不是必要的。此外，对投射的抑制并不影响偶然味觉学习本身，但在CTA非条件刺激（即不适）编码期间或之前，有效地抑制了厌恶味觉记忆的检索。值得注意的是，在没有经历任何内部不适的情况下，在新奇味觉消费后激活投射足以形成人工的厌恶味觉记忆，从而在检索时产生强烈的厌恶行为。我们的研究结果表明，aic - bla投射神经元是大脑将味觉刺激与负价体状态联系起来的能力的重要组成部分，并在味觉记忆检索中指导价特异性行为的表达。
Conditioned taste aversion (CTA) is an associative learning paradigm, wherein consumption of an appetitive tastant (e.g., saccharin) is paired to the administration of a malaise-inducing agent, such as intraperitoneal injection of LiCl. Aversive taste learning and retrieval require neuronal activity within the anterior insula (aIC) and the basolateral amygdala (BLA). Here, we labeled neurons of the aIC projecting to the BLA in adult male mice using a retro-AAV construct and assessed their necessity in aversive and appetitive taste learning. By restricting the expression of chemogenetic receptors in aIC-to-BLA neurons, we demonstrate that activity within the aICto-BLA projection is necessary for both aversive taste memory acquisition and retrieval, but not for its maintenance, nor its extinction. Moreover, inhibition of the projection did not affect incidental taste learning per se, but effectively suppressed aversive taste memory retrieval when applied either during or before the encoding of the unconditioned stimulus for CTA (i.e., malaise). Remarkably, activation of the projection after novel taste consumption, without experiencing any internal discomfort, was sufficient to form an artificial aversive taste memory, resulting in strong aversive behavior upon retrieval. Our results indicate that aIC-to-BLA projecting neurons are an essential component in the ability of the brain to associate taste sensory stimuli with body states of negative valence and guide the expression of valence-specific behavior upon taste memory retrieval.