TONIC RESPONSES TO EPIDERMAL GROWTH-FACTOR IN ZEBRAFISH CELLS
TONIC RESPONSES TO EPIDERMAL GROWTH-FACTOR IN ZEBRAFISH CELLS
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DOI：
10.1006/excr.1995.1146
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发表时间：
1995-05-01
影响因子：
3.7
通讯作者：
ZIVKOVIC, D
中科院分区：
文献类型：
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作者：
PEPPELENBOSCH, MP;TERTOOLEN, LGJ;ZIVKOVIC, D
在哺乳动物细胞中，对表皮生长因子（EGF）的最早细胞反应已被广泛表征，包括许多离子变化，例如钙流入的瞬时增加和膜超极化。这些离子变化的生理意义尚不确定。因此，确定这种离子变化在进化过程中是否保守非常重要，因为这表明离子信号在生长因子作用中发挥着不可或缺的作用。我们分离了几种胚胎斑马鱼细胞系，并研究了将 EGF 应用于这些细胞所引发的离子事件。使用全细胞膜片钳记录，我们表明这些受体的激活会诱导外向电流，该电流依赖于细胞外钙的流入。通过与不同的白三烯合成抑制剂预孵育，EGF诱导的跨膜电流被消除，这是EGF引发的离子信号传导的一个特征，也在哺乳动物细胞系中观察到。因此，这些结果表明离子信号传导以及潜在的第二信使系统并不限于哺乳动物细胞，表明离子信号传导在生长因子的作用中非常重要。 (C) 1995 学术出版社
In mammalian cells the earliest cellular responses to epidermal growth factor (EGF) have been extensively characterized and include a number of ionic changes, such as a transient increase in calcium influx and a membrane hyperpolarization. The physiological significance of these ionic changes is uncertain. Therefore it is important to establish whether such ionic changes have been conserved during evolution, as this would point to an indispensable role for ionic signaling in growth factor action. We have isolated several embryonic zebrafish cell lines and studied the ionic events elicited by application of EGF to these cells. Using whole cell patch clamp recording, we show that activation of these receptors induces an outward current, which is dependent on the influx of extracellular calcium, The EGF-induced transmembrane currents were abolished by preincubation with different inhibitors of leukotriene synthesis, a feature of EGF-provoked ionic signaling which is also observed in mammalian cell lines, Therefore, these results demonstrate that ionic signaling, as well as the underlying second-messenger systems, is not restricted to mammalian cells, indicating that ionic signaling is of importance in growth factor action. (C) 1995 Academic Press, Inc.