ENGINEERED IRON OXIDE-ADHESION MUTANTS OF THE ESCHERICHIA-COLI PHAGE-LAMBDA RECEPTOR
ENGINEERED IRON OXIDE-ADHESION MUTANTS OF THE ESCHERICHIA-COLI PHAGE-LAMBDA RECEPTOR
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DOI：
10.1073/pnas.89.18.8651
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发表时间：
1992-09-15
影响因子：
11.1
通讯作者：
BROWN, S
中科院分区：
文献类型：
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作者：
BROWN, S
通过基因工程构建了能够特异性粘附氧化铁而不粘附其他金属氧化物的大肠杆菌。将随机寡核苷酸的串联体引入编码噬菌体A受体外部结构域的质粒携带的lamB基因的一部分中。通过从质粒转化体群体中连续富集来选择能够粘附于氧化铁的细菌。插入DNA的多联体性质允许进行类似于外显子改组的遗传分析。这种遗传分析的结果表明，在一些分离物中，部分结合位点由侧翼载体序列编码。这种策略可能被证明通常用于鉴定能够识别特定表面的蛋白质序列。
Escherichia coli able to specifically adhere to iron oxide and not adhere to other metal oxides were constructed by genetic engineering. Concatamers of random oligonucleotides were introduced into a portion of a plasmid-borne lamB gene encoding an external domain of the phage A receptor. Bacteria able to adhere to iron oxide were selected by serial enrichment from the population of plasmid transformants. The concatameric nature of the inserted DNA allows a genetic analysis analogous to exon shuffling. Results of this genetic analysis indicate that in some isolates, part of the binding site is encoded by flanking vector sequences. This strategy may prove generally useful for identifying protein sequences able to recognize specific surfaces.