Thermodynamic study of the crystallization of sickle-cell deoxyhemoglobin (hemoglobin S solubility/saturation concentration/enthalpy of crystallization/entropy of crystallization).
Thermodynamic study of the crystallization of sickle-cell deoxyhemoglobin (hemoglobin S solubility/saturation concentration/enthalpy of crystallization/entropy of crystallization).
复制标题
镰状细胞脱氧血红蛋白结晶的热力学研究（血红蛋白S溶解度/饱和浓度/结晶热函/结晶熵）。
DOI：
10.1016/0022-2836(79)90061-5
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发表时间：
1979
影响因子：
5.6
通讯作者：
J. Steinhardt
中科院分区：
文献类型：
--
作者：
M. Jones;J. Steinhardt
用一种新的浊度法测定了结晶脱氧镰状细胞血红蛋白的溶解度。在没有变构效应剂六磷酸肌醇的情况下，镰刀形脱氧血红蛋白晶体的溶解度比凝胶的溶解度低约30%。在pH 7.1时晶体的溶解度对温度的依赖关系与在pH 6.48时凝胶的溶解度对温度的依赖关系非常相似。范特霍夫热随结晶的变化是正的，但变化很小(3.11kcal摩尔−1)，并且在30℃以下基本上与温度无关。在40cal K−1mol−1以下形成晶体时的熵变大于凝胶的ΔS°的值。所报告的测量结果都不需要估计颗粒体积和成分。
The solubility of crystalline deoxygenated sickle-cell hemoglobin has been determined by a new turbidometric technique. In the absence of the allosteric effector, inositol hexaphosphate, the solubility of sickle deoxyhemoglobin crystals is about 30% less than that of gels. The dependence of the solubility of crystals on temperature at pH 7.1 is very much the same as that of gels at pH 6.48. The change in Van't Hoff enthalpy with crystallization is positive but small (3.11 kcal mol−1) and essentially independent of temperature below 30 °C. The presence of inositol hexaphosphate cuts the solubility almost in half. The entropy change when crystals form, just under 40 cal K−1mol−1, is larger than the values ofΔS° found for gels. None of the measurements reported required the estimation of pellet volumes and compositions.