Lipidomic analysis of the liver from high-fat diet induced obese mice identifies changes in multiple lipid classes
Lipidomic analysis of the liver from high-fat diet induced obese mice identifies changes in multiple lipid classes
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DOI：
10.1016/j.yexmp.2014.05.002
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发表时间：
2014-08-01
影响因子：
3.6
通讯作者：
Buechler, Christa
中科院分区：
文献类型：
--
作者：
Eisinger, Kristina;Krautbauer, Sabrina;Buechler, Christa
脂肪肝与肥胖密切相关，并使肝脏对进一步的损伤变得敏感。本研究的目的是 1) 确定啮齿动物脂肪肝中脂质种类的变化，2) 分析这些脂质与脂肪肝中升高的甘油三酯、胆固醇或 CXCL8 之间可能的关联，3) 找出这些脂质的全身水平是否一致改变。脂质组学分析证实了已经报道的脂肪肝中磷脂酰胆碱的减少。磷脂酰丝氨酸较低，磷脂酰乙醇胺趋于减少。鞘磷脂水平正常，而单不饱和神经酰胺和己糖神经酰胺减少。总脂质部分中测量的 20 种脂肪酸中有 16 种升高，而 α-亚麻酸减少。值得注意的是，中链饱和脂肪酸显着减少。缩醛磷脂 18:0 和 18:1 种类在脂肪变性肝脏中强烈增加。没有一个显着变化的个体脂质种类与肝脏 CXCL8 mRNA、甘油三酯或胆固醇密切相关。脂肪肝中约 60% 的脂质改变在血清中也发生一致改变。这些数据表明，脂肪肝中的脂质成分存在多种变化，并且部分脂质可以通过血清分析来监测。 (C) 2014 Elsevier Inc. 保留所有权利。
Fatty liver is closely associated with obesity and sensitizes the liver to further insults. The aims of the current study are 1) to identify lipid species changed in rodent fatty liver, 2) to analyze for possible associations of these lipids with triglycerides, cholesterol or CXCL8 which is elevated in the steatotic liver and 3) to find out whether systemic levels of these lipids are concordantly altered. Lipidomic analysis has confirmed an already reported reduction of phosphatidylcholine in the steatotic liver. Phosphatidylserine is lower and phosphatidylethanolamine tends to be diminished. Sphingomyelin levels are normal while monounsaturated ceramides and hexosylceramides are reduced. Sixteen of the 20 fatty acid species measured in the total lipid fraction are elevated while alpha-linolenic acid is diminished. Of note, medium chain saturated fatty acids are markedly decreased. Plasmalogen 18:0 and 18:1 species are strongly increased in the steatotic liver. None of the markedly changed individual lipid species strongly correlates with hepatic CXCL8 mRNA, triglycerides or cholesterol. About 60% of the lipids altered in fatty liver are congruently altered in serum. These data show that there are multiple changes in lipid composition in fatty liver and part of the lipids may be monitored by serum analysis. (C) 2014 Elsevier Inc. All rights reserved.