Failure To Detect Helicobacter pylori DNA in Drinking and Environmental Water in Dhaka, Bangladesh, Using Highly Sensitive Real-Time PCR Assays
Failure To Detect Helicobacter pylori DNA in Drinking and Environmental Water in Dhaka, Bangladesh, Using Highly Sensitive Real-Time PCR Assays
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DOI：
10.1128/aem.02779-08
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发表时间：
2009-05-15
影响因子：
4.4
通讯作者：
Svennerholm, Ann-Mari
中科院分区：
文献类型：
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作者：
Janzon, Anders;Sjoling, Asa;Svennerholm, Ann-Mari
幽门螺杆菌的主要传播途径尚未确定，但有几份报告描述了H。pylori DNA的检测结果表明，饮用水和环境水中的H.幽门螺杆菌可能是水传播的。为了解决这种可能性，我们开发，测试和优化了两个互补的H。幽门特异性实时PCR测定用于H.水中的幽门螺旋杆菌DNA试验（包括采集程序和DNA提取）的最低检测水平约为250 H。每个水样的幽门螺杆菌基因组。使用我们的检测，然后，我们分析了饮用水和环境水（n = 75）和天然水生物膜（n = 21）从高流行地区在孟加拉国的样本。我们无法识别H。在任何样品中检测到幽门螺旋杆菌DNA，即使先前通过使用相同的方法在相同的水样中发现了其他致病细菌。进行了一系列对照实验，以确保阴性结果不是由PCR抑制、非特异性测定、模板DNA降解或低检测灵敏度引起的错误结果。我们的研究结果表明，这是不可能的主要传播途径的H。这个地区的幽门螺旋杆菌是通过水传播的
The main transmission pathway of Helicobacter pylori has not been determined, but several reports have described detection of H. pylori DNA in drinking and environmental water, suggesting that H. pylori may be waterborne. To address this possibility, we developed, tested, and optimized two complementary H. pylorispecific real-time PCR assays for quantification of H. pylori DNA in water. The minimum detection level of the assays including collection procedures and DNA extraction was shown to be approximately 250 H. pylori genomes per water sample. Using our assays, we then analyzed samples of drinking and environmental water (n = 75) and natural water biofilms (n = 21) from a high-endemicity area in Bangladesh. We could not identify H. pylori DNA in any of the samples, even though other pathogenic bacteria have been found previously in the same water samples by using the same methodology. A series of control experiments were performed to ensure that the negative results were not falsely caused by PCR inhibition, nonspecific assays, degradation of template DNA, or low detection sensitivity. Our results suggest that it is unlikely that the predominant transmission route of H. pylori in this area is waterborne.