ASPECTS OF SYNTHESIS OF POLIOVIRUS RNA AND FORMATION OF VIRUS PARTICLES
ASPECTS OF SYNTHESIS OF POLIOVIRUS RNA AND FORMATION OF VIRUS PARTICLES
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DOI：
10.1016/0042-6822(66)90024-9
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发表时间：
1966-01-01
期刊：
影响因子：
3.7
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
--
作者：
BALTIMORE, D;GIRARD, M;DARNELL, JE
在HeLa细胞中，脊髓灰质炎病毒RNA合成在感染后一小时内开始，并以指数形式持续3小时，此时速率变得线性。在感染2小时后，没有一种RNA进入病毒颗粒，但在多聚核糖体中发现了它。2.5小时后，RNA也开始被包裹在病毒颗粒中，4小时后，已合成的总RNA的20%在病毒中。在感染3.75小时后，RNA分子在合成后不到5分钟内就可以被病毒蛋白包裹，而且这种包裹可以在没有蛋白质合成的情况下发生。根据这些数据得出的结论是：(1)脊髓灰质炎病毒的“月食阶段”的概念仅在病毒颗粒形成方面有效。病毒RNA合成在感染后很早就开始了(2)在感染的早期阶段，负责病毒RNA合成的模板大量繁殖。RNA合成的指数上升不仅仅是由于输入分子中模板前体的激活不同步所致。(3)在感染细胞中存在病毒外壳蛋白前体。给定病毒颗粒的所有蛋白质都可以在其RNA之前合成。
In HeLa cells, poliovirus RNA synthesis begins within an hour after infection and continues exponentially for 3 hours, the rate becoming linear at this time. Up to 2 hours of infection, none of the RNA which has been made has entered virus particles but is found in polyribosomes. After 2.5 hours, RNA begins also to be enclosed within virus particles, and by 4 hours, 20% of the total RNA which has been synthesized is in virus. At 3.75 hours of infection, an RNA molecule can be coated with viral protein within less than 5 minutes after its synthesis, and this coating can take place in the absence of protein synthesis. Conclusions drawn from these data are: (1) The concept of an "eclipse phase" for poliovirus is valid only in terms of virus particle formation. Virus RNA synthesis commences very early after infection (2) The templates reponsible for viral RNA synthesis multiply during the early stage of infection. The exponential rise in RNA synthesis is not due simply to asynchrony of activation of template precursors among the input molecules. (3) There exists in the infected cell a pool of viral coat-protein precursors. All the protein for a given virus particle can be synthesized prior to its RNA.