Recombinant feline leukemia virus genes detected in naturally occurring feline lymphosarcomas.
Recombinant feline leukemia virus genes detected in naturally occurring feline lymphosarcomas.
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在自然发生的猫淋巴肉瘤中检测到的重组猫白血病病毒基因。
DOI：
10.1128/jvi.67.6.3118-3125.1993
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发表时间：
1993
影响因子：
5.4
通讯作者：
Roy-Burman,P
中科院分区：
文献类型：
--
作者：
Sheets,RL;Pandey,R;Jen,WC;Roy-Burman,P
使用旨在检测重组猫白血病病毒 (FeLV) 基因组的聚合酶链反应策略，该策略具有源自内源来源的 5' env 序列和来自 FeLV A 亚组 (FeLV-A) 的 3' env 序列，我们在大约四分之三的天然胸腺和消化性猫淋巴肉瘤 (LSA) 和三分之一确定为 FeLV 衣壳抗原的猫多中心 LSA 中检测到重组原病毒免疫荧光检测呈阳性。相比之下，22个自然产生的FeLV阴性猫LSA中只有1个含有重组原病毒，并且在来自正常组织或死于其他疾病的FeLV阳性动物组织的7个样本中没有检测到重组env基因。在重组体中鉴定出了四个优选的结构基序；一种是 FeLV-B 样（认识到 FeLV-B 本身是 FeLV-A 和内源性 env 基因之间重组的产物），三种在转换为 FeLV-A 相关序列之前含有不同数量的内源性 env 基因：（i）全长和删除的 env 基因的组合，在表面糖蛋白（SU）中间位点进行重组，（ii）由内源性样序列编码的整个 SU，以及（iii）整个SU和大约一半的跨膜蛋白由内源样序列编码。此外，三个胸腺肿瘤含有重组原病毒，其 SU 蛋白主要中和决定簇附近发生突变。这些 LSA DNA 的分子遗传学分析与我们之前的体外结果相对应，并支持 FeLV 诱导的白血病发生中体内病毒重组体和突变体的发生和关联。
Using a polymerase chain reaction strategy aimed at detecting recombinant feline leukemia virus (FeLV) genomes with 5' env sequences originating from an endogenous source and 3' env sequences resulting from FeLV subgroup A (FeLV-A), we detected recombinant proviruses in approximately three-fourths of naturally occurring thymic and alimentary feline lymphosarcomas (LSAs) and one-third of the multicentric LSAs from cats determined to be FeLV capsid antigen positive by immunofluorescence assay. In contrast, only 1 of 22 naturally arising FeLV-negative feline LSAs contained recombinant proviruses, and no recombinant env gene was detected in seven samples from normal tissues or tissues from FeLV-positive animals that died from other diseases. Four preferred structural motifs were identified in the recombinants; one is FeLV-B like (recognizing that FeLV-B itself is a product of recombination between FeLV-A and endogenous env genes), and three contain variable amounts of endogenous-like env gene before crossing over to FeLV-A-related sequences: (i) a combination of full-length and deleted env genes with recombination at sites in the middle of the surface glycoprotein (SU), (ii) the entire SU encoded by endogenous-like sequences, and (iii) the entire SU and approximately half of the transmembrane protein encoded by endogenous-like sequences. Additionally, three of the thymic tumors contained recombinant proviruses with mutations in the vicinity of the major neutralizing determinant for the SU protein. These molecular genetic analyses of the LSA DNAs correspond to our previous results in vitro and support the occurrence and association of viral recombinants and mutants in vivo in FeLV-induced leukemogenesis.