A G-box motif (GCCACGTGCC) tetramer confers high-level constitutive expression in dicot and monocot plants
A G-box motif (GCCACGTGCC) tetramer confers high-level constitutive expression in dicot and monocot plants
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DOI：
10.1046/j.1365-313x.1999.00456.x
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发表时间：
1999-05-01
期刊：
影响因子：
7.2
通讯作者：
Oeda, K
中科院分区：
文献类型：
--
作者：
Ishige, F;Takaichi, M;Oeda, K
分析了11个具有G-box核心的基础启动子（CaMV-90） （CACGTG）的GUS报告基因表达，以评价G-box侧翼序列的调控作用。虽然大多数G-box基序表现出一定的组织偏好基因表达，但明显的组织特异性表达并不明显。然而，在11个G-box序列中，G-box 10 （GCCACGTGCC）四聚体在转基因烟草的种子、根、叶、腋芽、花蕾的几乎所有部位和花粉中都有高水平的组成表达。G-box - 10四聚体启动子在转基因双头胡萝卜和单子稻中均有高水平表达。这显然是第一个关于G-box基序以非组织特异性方式赋予高水平本构表达的报道。
GUS reporter expression from 11 basal promoters (CaMV-90) with G-box cores (CACGTG) was analysed to evaluate the regulatory roles of G-box flanking sequences. While most G-box motifs exhibited some tissue preference of gene expression, a distinct tissue-specific expression was not apparent. However, one of 11 G-box sequences, the G-box 10 (GCCACGTGCC) tetramer, conferred a high-level constitutive expression in seed, root, leaf, axillary bud, almost all parts of flower buds and pollen of transgenic tobacco plants. Furthermore, the G-box 10 tetramer promoter exhibited high-level expression in transgenic dicot carrot and monocot rice. This is apparently the first report of a G-box motif conferring a high-level constitutive expression in a non-tissue-specific manner.