Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet
Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet
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DOI：
10.1101/2023.02.02.526762
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发表时间：
2023-12
期刊：
影响因子：
7.7
通讯作者：
Carla de la Fuente;A. Grondin;B. Sine;M. Debieu;C. Belin;A. Hajjarpoor;J. Atkinson;S. Passot;Marine Salson;Julie Orjuela;Christine Tranchant-Dubreuil;Jean‐Rémy Brossier;Maxime Steffen;Charlotte Morgado;Hang Ngan Dinh;Bipin K. Pandey;Julie Darmau;A. Champion;A. Petitot;Célia Barrachina;M. Pratlong;Thibault Mounier;Princia Nakombo-Gbassault;P. Gantet;P. Gangashetty;Y. Guédon;V. Vadez;J. Reichheld;M. Bennett;N. Kane;Soazig Guyomarc’h;D. Wells;Y. Vigouroux;L. Laplaze
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文献类型：
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作者：
Carla de la Fuente;A. Grondin;B. Sine;M. Debieu;C. Belin;A. Hajjarpoor;J. Atkinson;S. Passot;Marine Salson;Julie Orjuela;Christine Tranchant-Dubreuil;Jean‐Rémy Brossier;Maxime Steffen;Charlotte Morgado;Hang Ngan Dinh;Bipin K. Pandey;Julie Darmau;A. Champion;A. Petitot;Célia Barrachina;M. Pratlong;Thibault Mounier;Princia Nakombo-Gbassault;P. Gantet;P. Gangashetty;Y. Guédon;V. Vadez;J. Reichheld;M. Bennett;N. Kane;Soazig Guyomarc’h;D. Wells;Y. Vigouroux;L. Laplaze
幼苗根系性状影响着逆境下植物的生长。珍珠粟是最耐热和最耐旱的谷类作物之一，为撒哈拉以南萨赫勒地区提供了重要的食物来源。珍珠粟的早期根系特征是单一的快速生长的主根，我们假设这是对萨赫勒气候的适应。利用作物模型，我们证明了早期干旱胁迫是萨赫勒地区种植珍珠粟的农业系统的一个重要制约因素。此外，我们还表明，珍珠粟初生根生长的增加与田间条件下早期对水分胁迫的耐受性增加有关。遗传学包括GWAS和QTL方法鉴定控制这一关键根性状的基因组区域。结合基因表达数据，对其中一个基因组区域进行重新测序和重新注释，确定了glutaredoxin编码基因PgGRXC9作为胁迫抗逆性根生长调节因子的候选基因。其与拟南芥同源物AtROXY19的功能表征揭示了glutaredoxin （GRX）基因分支在调节细胞伸长中的新作用。综上所述，我们的研究表明GRX基因在赋予珍珠粟根细胞伸长和增强其对萨赫勒环境的恢复力方面具有保守功能。
Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet’s early root system features a single fast-growing primary root which we hypothesize is an adaptation to the Sahelian climate. Using crop modelling, we demonstrate that early drought stress is an important constraint in agrosystems in the Sahel where pearl millet was domesticated. Furthermore, we show that increased pearl millet primary root growth is correlated with increased early water stress tolerance in field conditions. Genetics including GWAS and QTL approaches identify genomic regions controlling this key root trait. Combining gene expression data, re-sequencing and re-annotation of one of these genomic regions identified a glutaredoxin-encoding gene PgGRXC9 as the candidate stress resilience root growth regulator. Functional characterization of its closest Arabidopsis homolog AtROXY19 revealed a novel role for this glutaredoxin (GRX) gene clade in regulating cell elongation. In summary, our study suggests a conserved function for GRX genes in conferring root cell elongation and enhancing resilience of pearl millet to its Sahelian environment.