Antibodies to liver cell adhesion molecule perturb inductive interactions and alter feather pattern and structure.
Antibodies to liver cell adhesion molecule perturb inductive interactions and alter feather pattern and structure.
复制标题
肝细胞粘附分子的抗体会扰乱诱导相互作用并改变羽毛图案和结构。
DOI：
10.1073/pnas.83.21.8235
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Edelman,GM
中科院分区：
文献类型：
--
作者：
Gallin,WJ;Chuong,CM;Finkel,LH;Edelman,GM
细胞粘附分子（CAM）可以通过限制细胞运动、形成边界和控制导致胚胎诱导的细胞间通讯来充当形态发生的调节剂。这一假设的因果作用CAM诱导事件进行了测试，在体外系统的羽毛诱导。在发育中的鸡皮肤中，外胚层上皮层与中胚层细胞集合体相互作用，形成或多或少呈六边形排列的圆形羽芽。表皮上皮细胞通过肝CAM（L-CAM）连接，真皮致密物中的中胚层细胞通过神经CAM（N-CAM）连接;这些CAM都不将这种诱导偶联的一个组织中的细胞与另一个组织中的细胞连接。扰动后的L-CAM链接在表皮中的L-CAM抗体，非六边形条纹图案的真皮冷凝在文化中观察到。条纹不是直线，而是在横向和倾斜方向上蜿蜒。扰动组织的组织学检查显示真皮细胞密度分布的广泛变化。培养10天后，扰动的组织形成类似鳞片的鹅卵石或斑块状形态，而不是在未扰动的皮肤培养物中形成的羽毛状丝状结构。结果表明，干扰CAM结合在组织中连接的一个CAM可以改变命运和相互作用的细胞连接的另一个，大概是通过改变诱导信号的量或效果之间的诱导细胞集体跨越边界。一个计算机模型的基础上的概念，L-CAM连接的表皮细胞的信号从N-CAM连接的真皮细胞的反应取决于合作的程度L-CAM连接被发现产生六边形图案的未扰动的情况下和条纹扰动后的L-CAM债券。
Cell adhesion molecules (CAMs) may act as regulators of morphogenesis by constraining cell motion, forming borders, and controlling intercellular communications that lead to embryonic induction. This postulated causal role of CAMs in inductive events was tested here in an in vitro system of feather induction. In the developing chicken skin, an ectodermal sheet of epithelium interacts with mesodermal cell collectives to form more or less circular feather germs arranged in a hexagonal pattern. Cells of the epidermal epithelium are linked by liver CAM (L-CAM) and mesodermal cells in dermal condensations are linked by neural CAM (N-CAM); neither of these CAMs links cells in one tissue of this inductive couple to cells in the other. After perturbation of the L-CAM linkage in epidermis by antibodies to L-CAM, nonhexagonal striped patterns of dermal condensations were observed in culture. The stripes did not follow straight lines but meandered in lateral and oblique directions. Histological examination of the perturbed tissues showed extensive changes in dermal cell density distributions. After 10 days of culture, the perturbed tissues developed a cobbled or plaque-like morphology resembling scales rather than the feather-like filamentous structures that formed in unperturbed skin cultures. The results indicate that perturbation of CAM binding in tissues linked by one CAM can alter fates and interactions of cells linked by another, presumably by altering the amount or effect of inductive signals crossing the border between the inducing cell collectives. A computer model based on the notion that the response of L-CAM-linked epidermal cells to signals from N-CAM-linked dermal cells depends cooperatively on the degree of L-CAM linkage was found to generate hexagonal patterns for the unperturbed case and stripes after perturbation of L-CAM bonds.