Decreased bacteria density on nanostructured polyurethane.
Decreased bacteria density on nanostructured polyurethane.
复制标题
DOI：
10.1002/jbm.a.34856
复制
发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
C. Yao;T. Webster;M. Hedrick
中科院分区：
文献类型：
--
作者：
C. Yao;T. Webster;M. Hedrick
众所周知，医疗器械感染是一个日益严重的临床问题，由于之前使用抗生素降低细菌功能的尝试失败，细菌很快对其产生了耐药性，因此没有明确的解决方案。由于它们的表面能量发生了改变，本体外研究的目的是通过将 PU 膜浸泡在 HNO3 中来在聚氨酯 (PU) 上创建纳米级表面特征，并确定 1 小时后细菌（特别是表皮葡萄球菌、大肠杆菌和奇异变形杆菌）菌落形成单位。此类细菌经常感染许多医疗设备。结果提供了第一个证据，即在不使用抗生素的情况下，与传统 PU 和组织工程对照小肠粘膜下层 (SIS) 相比，表皮葡萄球菌密度分别降低了 5 倍和 13 倍，大肠杆菌密度降低了 6 倍和 20 倍，奇异果密度降低了 8 倍和 35 倍。材料表征研究表明，与传统 PU（尽管仍然是亲水性的）相比，经 HNO3 处理的纳米结构 PU 具有显着更大的纳米级粗糙度和疏水性，这可能为观察到的细菌反应降低提供了理论依据。此外，与传统 PU 相比，在纳米粗糙表面上测量到的血清纤连蛋白吸附量显着增加，这可能解释了细菌生长的减少。总之，这项研究为使用纳米结构聚氨酯在不使用抗生素的情况下降低细菌功能提供了重要前景，明确解决了当今观察到的医疗器械感染增加的广泛传播问题。
As is well known, medical device infections are a growing clinical problem with no clear solution due to previous failed attempts of using antibiotics to decrease bacteria functions for which bacteria quickly develop a resistance toward. Because of their altered surface energetics, the objective of the present in vitro study was to create nanoscale surface features on polyurethane (PU) by soaking PU films in HNO3 and to determine bacteria (specifically, S. epidermidis, E. coli, and P. mirabilis) colony forming units after 1 h. Such bacteria frequently infect numerous medical devices. Results provided the first evidence that without using antibiotics, S. epidermidis density decreased by 5 and 13 times, E. coli density decreased by 6 and 20 times, and P. mirabilis density decreased by 8 and 35 times compared to conventional PU and a tissue engineering control small intestine submucosa (SIS), respectively. Material characterization studies revealed significantly greater nanoscale roughness and hydrophobicity for the HNO3-treated nanostructured PU compared to conventional PU (albeit, still hydrophilic) which may provide a rationale for the observed decreased bacteria responses. In addition, significantly greater amounts of fibronectin adsorption from serum were measured on nanorough compared conventional PU which may explain the decreased bacteria growth. In summary, this study provides significant promise for the use of nanostructured PU to decrease bacteria functions without the use of antibiotics, clearly addressing the wide spread problem of increased medical device infections observed today.