Assembling drug-loaded-layered double hydroxide nanohybrids with poloxamer 188 for improved cellular uptake and in vitro efficacy
Assembling drug-loaded-layered double hydroxide nanohybrids with poloxamer 188 for improved cellular uptake and in vitro efficacy
复制标题
用泊洛沙姆 188 组装载药层状双氢氧化物纳米杂化物以改善细胞摄取和体外功效
DOI：
10.1557/s43578-022-00813-w
复制
发表时间：
2022
影响因子：
2.7
通讯作者：
Jingmou Yu
中科院分区：
文献类型：
--
作者：
Jin Ren;Liang Liang;Yongqing Yang;Xiang Liu;Weidong Li;Wenbo Liu;Yali Wang;Jingmou Yu
层状双氢氧化物（LDHs）需要表面功能化以实现有效药物递送所需的胶体和生物稳定性。本工作报道了一种新的功能化方法，构建负载药物的纳米杂化物（2：1E和2.8：1E）通过共组装LDH片，五氟脲嘧啶（5 Fu）和泊洛沙姆188通过剥离重组。一些分析表明，制备适当粒径的2：1E比2.8：1E需要更多的泊洛沙姆188。与离子交换法相比，2.8：1E法在载药量和分散性方面具有优势。此外，这些纳米杂化物可以改善裸载药LDH中5 Fu的突然释放。最重要的是，与裸载药LDH相比，在这些纳米杂化物中观察到显著增强的细胞摄取和更好的细胞毒性。最后，结果表明，组装载药LDH与泊洛沙姆188是一个有前途的策略，以提高细胞摄取和体外功效的LDH的生物医学应用。通过剥离重组法将LDH片层、五氟脲嘧啶（5Fu）和泊洛沙姆188组装成载药纳米复合物，与离子交换法制备的裸载药LDH相比，具有分散性好、细胞摄取能力强、细胞毒性好等优点。
Layered Double Hydroxide (LDHs) requires surface functionalization to achieve desirable colloidal and biological stability for effective drug delivery. The present work reported a new functionalized approaches to construct drug-loaded nanohybrids (2:1E and 2.8:1E) by co-assembling LDH sheets, penta-fluorouracil (5Fu) and poloxamer 188 through exfoliation reassembling. Some analysis showed that more poloxamer 188 were needed to prepare 2:1E with appropriate particle size than 2.8:1E. Besides, 2.8:1E shows advantages in drug loading rate and dispersion as compared with ion exchange method. In addition, these nanohybrids can improve the sudden release of 5Fu in naked drug-loaded LDH. Most importantly, significant enhanced cell uptake and better cytotoxicity were seen in these nanohybrids in contrast to naked drug-loaded LDH. Lastly, the results reveal that assembling drug-loaded LDH with poloxamer 188 is a promising strategy to improve cellular uptake and in vitro efficacy of LDHs for biomedical applications. Graphical abstract Drug-loaded nanohybrids were synthesised by co-assembling LDH sheets, penta-fluorouracil (5Fu) and poloxamer 188 through exfoliation reassembling, and showed advantages in dispersion, enhanced cell uptake and better cytotoxicity as compared with naked drug-loaded-LDH prepared by ion exchange method.