Protective effects of tryptophan on neuro-inflammation in rats after administering lipopolysaccharide
Protective effects of tryptophan on neuro-inflammation in rats after administering lipopolysaccharide
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DOI：
10.1016/j.biopha.2011.02.008
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发表时间：
2011-06-01
影响因子：
7.5
通讯作者：
Bitzer-Quintero, O. K.
中科院分区：
文献类型：
--
作者：
Del Angel-Meza, A. R.;Davalos-Marin, A. J.;Bitzer-Quintero, O. K.
色氨酸（Tryptophan，TRP）是一种内源性的自由基清除剂和抗氧化剂，在免疫调节、蛋白质合成、5-羟色胺（5-HT）和褪黑激素（melatonin）的产生等方面发挥重要作用。本工作的目的是确定TRP在全身给予脂多糖（LPS，20 mg/kg）诱导的神经炎症中的功效，脂多糖（LPS，20 mg/kg）促进自由基（LPO：MDA和4-HDA）和促炎细胞因子干扰素-γ（IFN-γ）在大鼠不同脑区域（大脑皮层和海马）中的合成。对喂食含TRP（0.5 mg/100 g蛋白质）饲料的成年雌性、妊娠和哺乳期大鼠进行实验，评价大脑皮层和海马的脂质过氧化（LPO）产物、亚硝酸盐、硝酸盐和IFN-γ的血浆浓度。LPS + TRP组LPO水平较LPS组明显降低。然而，在大脑皮层或海马中，亚硝酸盐和硝酸盐以及IFN-γ的血浆水平没有观察到差异。TRP作为自由基清除剂，对内毒素休克模型中的氧化损伤具有保护作用。因此，可作为内毒素休克过程中的一种无毒保护剂。(C)2011年Elsevier Masson SAS。All rights reserved.
Tryptophan (TRP), which plays an important role in immune system regulation, protein synthesis, serotonin (5-HT) and melatonin production, is a potent endogenous free radical scavenger and antioxidant. The aim of this work was to determine the efficacy of TRP in neuro-inflammation induced by systemic administration of lipopolysacharide (LPS, 20 mg/kg) which promotes the synthesis of free radical (LPO: MDA and 4-HDA), and pro-inflammatory cytokine Interferon-gamma (IFN-gamma) in different brain regions (cerebral cortex and hippocampus) of rats. Experiments were performed on adult female, pregnant and lactating rats fed with a diet of TRP content (0.5 mg/100 g protein), cerebral cortex and hippocampus were evaluated for lipid peroxidation (LPO) products, nitrites, nitrates and plasmatic concentration of IFN-gamma. LPO levels in LPS + TRP groups were significantly decreased than that obtained in the LPS group. However, there were no observed differences in plasmatic levels of nitrites and nitrates as well as IFN-gamma, neither in the cerebral cortex or hippocampus. The TRP has protective effect in the oxidative damage in a model of endotoxic shock in the breading nurslings induced by the systemic administration of LPS, acting as a scavenger of free radicals. So, it can be proposed as an innocuous protector agent in the endotoxic shock process. (C) 2011 Elsevier Masson SAS. All rights reserved.