STRATEGIES FOR IMPROVED TARGETING OF THERAPEUTIC CELLS: IMPLICATIONS FOR TISSUE REPAIR
STRATEGIES FOR IMPROVED TARGETING OF THERAPEUTIC CELLS: IMPLICATIONS FOR TISSUE REPAIR
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DOI：
10.22203/ecm.v023a24
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Murphy, Mary
中科院分区：
文献类型：
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作者：
Ansboro, Sharon;Greiser, Udo;Murphy, Mary
多能间充质干细胞(MSCs)由于具有向软骨和成骨方向分化的能力，被认为是治疗骨关节炎等疾病的合适的细胞来源。MSCs可以从各种组织来源获得，可用于大规模生产，并且具有免疫特权，能够用于同种异体细胞治疗。然而，最近的临床前研究和临床试验表明，增加MSCs的植入和疗效是必要的。这篇综述探讨了细胞表面修饰如何改善骨髓间充质干细胞的归巢，并总结了纳米颗粒的使用，以实现干细胞的基因传递以及促进体内成像。讨论了先进生物材料的使用以及如何在治疗干预期间应用它们来减少MSCs的总剂量，同时实现细胞对病变部位的最佳靶向效率。特别关注改善MSCs植入软骨的方法，并综述了描述基于颗粒的细胞疗法的组合方法以促进软骨组织再生的研究。这种方法的使用将增加治疗骨关节炎的潜在再生疗法的阵列。
Multipotent mesenchymal stem cells (MSCs) have been suggested as a suitable cell source for cell-based treatments for diseases such as osteoarthritis due to their ability to differentiate towards chondrogenic and osteogenic lineages. MSCs can be obtained from a variety of tissue sources, are scalable for mass-production and immuno-privileged enabling their use for allogeneic cell therapy. However, recent pre-clinical studies and clinical trials point to the necessity of increasing engraftment and efficacy of MSCs. This review explores how cell surface modification of the cells can improve homing of MSCs and summarises the use of nanoparticles to enable gene delivery by stem cells as well as facilitate in vivo imaging. The use of advanced biomaterials and how they can be applied to reduce the overall dose of MSCs during therapeutic interventions while achieving optimal targeting efficiency of cells to the diseased sites are addressed. Particular attention is paid to methods that improve engraftment of MSCs to cartilage and research describing combinatorial approaches of particle-based cell therapies for improved regeneration of this tissue is reviewed. The use of such approaches will add to the array of potential regenerative therapeutics for treatment of osteoarthritis.