Highly divergent dengue virus type 1 genotype sets a new distance record
Highly divergent dengue virus type 1 genotype sets a new distance record
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DOI：
10.1038/srep22356
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发表时间：
2016-02-29
影响因子：
4.6
通讯作者：
van den Hurk, Andrew F.
中科院分区：
文献类型：
--
作者：
Pyke, Alyssa T.;Moore, Peter R.;van den Hurk, Andrew F.
登革病毒(DENV)是人类蚊媒病毒性疾病的主要原因。它们既存在于地方性生态型，也存在于森林生态型中。2014年，一名最近访问过文莱热带雨林的病毒症患者返回澳大利亚，表现出与DENV感染一致的症状。随后从患者身上分离出一株独特的DENV毒株，我们认为该毒株属于DENV血清1型(DENV-1)中的一个新基因型。贝叶斯进化进化树分析表明，推测的DENV-1文莱2014(文莱2014)是迄今记录的最具差异性的DENV-1，并将DENV-1到最近共同祖先(MRCA)的时间从大约120年增加到大约315年。对Brun2014毒株的DENV-1分类得到了单抗血清分型数据的进一步支持。表型特征表明，Brun2014在蚊子细胞中的复制率和埃及伊蚊的感染率与流行的DENV-1毒株没有显著差异。鉴于它有能力引起人类疾病并感染Ae。不能忽视埃及伊蚊、潜在的城市外溢和因2014年的进一步传播而导致的临床疾病。
Dengue viruses (DENVs) are the leading cause of mosquito-borne viral disease of humans. They exist in both endemic and sylvatic ecotypes. In 2014, a viremic patient who had recently visited the rainforests of Brunei returned to Australia displaying symptoms consistent with DENV infection. A unique DENV strain was subsequently isolated from the patient, which we propose belongs to a new genotype within DENV serotype 1 (DENV-1). Bayesian evolutionary phylogenetic analysis suggests that the putative sylvatic DENV-1 Brunei 2014 (Brun2014) is the most divergent DENV-1 yet recorded and increases the time to the most recent common ancestor (MRCA) for DENV-1 from approximate to 120 years to approximate to 315 years. DENV-1 classification of the Brun2014 strain was further supported by monoclonal antibody serotyping data. Phenotypic characterization demonstrated that Brun2014 replication rates in mosquito cells and infection rates in Aedes aegypti mosquitoes were not significantly different from an epidemic DENV-1 strain. Given its ability to cause human illness and infect Ae. aegypti, potential urban spillover and clinical disease from further Brun2014 transmission cannot be discounted.