AFM of biological material embedded in epoxy resin
AFM of biological material embedded in epoxy resin
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DOI：
10.1016/j.jsb.2004.01.010
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发表时间：
2004-06-01
影响因子：
3
通讯作者：
Mueller, M
中科院分区：
文献类型：
--
作者：
Matsko, N;Mueller, M
我们提出了一种简单的方法来提取形态细节的嵌段面环氧树脂嵌入的生物聚合物的AFM。它示出，地形对比度和识别的小结构的细节，关键取决于程序的样品制备前嵌入（化学固定或高压冷冻和冷冻取代）和嵌入环氧树脂的硬度。切片后，对样品块面进行乙醇处理，洗脱非交联聚合物链，使包埋生物材料的最小细节易于检测。AFM（高度和相位对比）检查相应制备的秀丽隐杆线虫细胞的块面提供了与TEM相当的数据。(C)2004爱思唯尔公司All rights reserved.
We present a simple method to extract morphological details from the block face of epoxy embedded biopolymers by AFM. It is shown that topographical contrast and the identification of small structural details critically depend on the procedure of sample preparation before embedding (chemical fixation or high-pressure freezing and freeze-substitution) and on the hardness of the embedding epoxy resin. Ethanol treatment of the block face of the sample after microtomy elutes non-cross-linked polymer chains and makes the smallest details of the embedded biomaterial amenable to detection. AFM (height and phase contrast) examination of the block face of accordingly prepared cells of Caenorhabditis elegans provides data that are comparable to TEM. (C) 2004 Elsevier Inc. All rights reserved.