Evaluation of the parameters determining the performance of electrochromatography in packed capillary columns
Evaluation of the parameters determining the performance of electrochromatography in packed capillary columns
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DOI：
10.1016/0021-9673(95)00718-3
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发表时间：
1995-11
影响因子：
4.1
通讯作者：
B. Behnke;E. Grom;E. Bayer
中科院分区：
文献类型：
--
作者：
B. Behnke;E. Grom;E. Bayer
电色谱法是在毛细管柱中进行的反相液相色谱法的一种变体，其中洗脱液的输送通过在柱的长度上施加电场来完成。在电色谱中，由于传统泵送遇到的流动阻力而引起的柱长限制是不存在的，允许在填充有1.5 μm固定相的毛细管中进行分离，最长可达50 cm，从而使每柱的效率超过100 000个板。然而，实际问题限制了所报告的成功申请的数量。一些参数决定的电色谱性能进行了研究：洗脱液，玻璃料，填充柱和可选的补充压力。这些因素被单独研究，然后逐步组合。使用彻底脱气的洗脱液、由烧结硅胶制成的玻璃料和使用辅助压力，可以容易地获得稳定和可再现的条件。
Electrochromatography is a varient of reversed-phase liquid chromatography performed in capillary columns whereby transport of the eluent is accomplished by applying an electric field across the length of the column. Column-length restrictions arising from resistance to flow encountered with conventional pumping are absent in electrochromatography, allowing separation in capillaries packed with 1.5-μm stationary phases up to 50 cm, thus rendering efficiencies of more than 100 000 plates per column. Practical problems, however, have restricted the number of successful applications reported. A number of parameters determining the electrochromatographic performance are investigated: eluent, frits, packed columns and optional supplementary pressure. These factors are investigated separately and then combined stepwise. With thoroughly degassed eluent, frits made of sintered silica gel, and the use of supplementary pressure, stable and reproducible conditions may be readily obtained.