Preparation of Slice Cultures from Rodent Hippocampus.
Preparation of Slice Cultures from Rodent Hippocampus.
复制标题
DOI：
10.1101/pdb.prot094888
复制
发表时间：
2017-02-01
影响因子：
--
通讯作者：
Oertner, Thomas G
中科院分区：
文献类型：
--
作者：
Gee, Christine E;Ohmert, Iris;Oertner, Thomas G
本方案描述了使用不需要使用抗生素或抗真菌剂的无菌条件从大鼠或小鼠幼仔制备海马切片培养物。结合非常好的光学和电生理的可及性与接近完整动物的寿命，许多关于突触可塑性和网络连接的长期动力学的基本问题可以用这种制剂来解决。
This protocol describes the preparation of hippocampal slice cultures from rat or mouse pups using sterile conditions that do not require the use of antibiotics or antimycotics. Combining very good optical and electrophysiological accessibility with a lifetime approaching that of the intact animal, many fundamental questions about synaptic plasticity and long-term dynamics of network connectivity can be addressed with this preparation.