EFFECT OF AMINO ACIDS AND OXYGEN ON CHEMOTAXIS IN ESCHERICHIA COLI
EFFECT OF AMINO ACIDS AND OXYGEN ON CHEMOTAXIS IN ESCHERICHIA COLI
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DOI：
10.1128/jb.92.1.121-129.1966
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
ADLER, J
中科院分区：
文献类型：
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作者：
ADLER, J
将大肠杆菌的能动细胞放置在含有蛋白质中常见的 20 种氨基酸混合物的毛细管一端，以两条带的形式迁移到管中。这些条带肉眼清晰可见，也可以通过显微镜、摄影、光密度测定以及整个试管中的细菌分析来证明。多个条带的出现并不是由于细菌之间的异质性，因为每个条带都可以重复使用以再次产生两条条带。第一条带使用所有氧气来氧化各种可氧化氨基酸的部分，包括丝氨酸，第二条带则厌氧消耗剩余的丝氨酸。结果解释为大肠杆菌对氧和丝氨酸表现出趋化性。当培养基中不添加能源时，仍然会出现一条细菌带。它消耗所有氧气来氧化内源能源。添加 10 种可氧化氨基酸中的任何一种都会刺激该带的移动速率。丙氨酸是一个经过详细研究的例子，它支持消耗所有氧气来氧化部分丙氨酸的带。丝氨酸是该菌株唯一可以在需氧或厌氧条件下使用的氨基酸，产生两条带。
Motile cells of E. coli placed at one end of a capillary tube containing a mixture of the 20 amino-acids commonly found in proteins migrate out into the tube in two bands. The bands are clearly visible to the naked eye, and they can also be demonstrated by microscopy, photography, and densitometry, and by assaying for bacteria throughout the tube. The occurrence of more than one band is not due to heterogeneity among the bacteria, since each band can be used over to give rise to two again. The first band uses all the oxygen to oxidize portions of the various oxidizable amino-acids, including serine, and the second band consumes the residual serine anaerobically. The results are interpreted to mean that E. coli shows chemotaxis toward oxygen and serine. When no energy source is added to the medium, a band of bacteria still appears. It consumes all the oxygen to oxidize an endogenous energy source. The addition of any one of 10 oxidizable amino-acids stimulates the rate of travel of this band. Alanine, an example that was studied in detail, supports such a band that consumes all the oxygen to oxidize a portion of the alanine. Serine, the only amino-acid that this strain can use either aerobically or anaerobically, gives rise to two bands.