In situ visualization of DNA double-strand break repair in human fibroblasts
In situ visualization of DNA double-strand break repair in human fibroblasts
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DOI：
10.1126/science.280.5363.590
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发表时间：
1998-04-24
期刊：
影响因子：
56.9
通讯作者：
Petrini, JHJ
中科院分区：
文献类型：
--
作者：
Nelms, BE;Maser, RS;Petrini, JHJ
开发了一种检测完整细胞内DNA修复的方法。使用超软x射线在人成纤维细胞的特定亚核体积中诱导DNA双链断裂（DSBs），并在这些部位观察DNA修复。在DNA修复的初始阶段，DSB保持在固定位置，DSB修复蛋白hMre11在30分钟内迁移到损伤部位。相比之下，人类RecA同源物hRad51并不定位于DNA损伤位点，这一发现与这些蛋白质在DNA修复中的独特作用一致。
A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.