Potential adverse effects of antimicrobial chemotherapy based on ultraviolet-A irradiation of polyphenols against the oral mucosa in hamsters and wounded skin in rats.
Potential adverse effects of antimicrobial chemotherapy based on ultraviolet-A irradiation of polyphenols against the oral mucosa in hamsters and wounded skin in rats.
复制标题
基于多酚紫外线 A 照射的抗菌化疗对仓鼠口腔粘膜和大鼠受伤皮肤的潜在副作用。
DOI：
10.1016/j.jphotobiol.2018.08.006
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Niwano Y.
中科院分区：
文献类型：
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作者：
Nakamura K;Tenkumo T;Mokudai T;Shirato M;Ishiyama K;Kanno T;Sasaki K;Niwano Y.
基于多酚溶液的UVA照射的抗菌化学疗法已被提出作为龋齿的预防性治疗。然而，这种治疗的安全性尚未得到全面评估。因此，本研究的目的是评估该处理对仓鼠口腔粘膜和大鼠创伤皮肤的影响。在仓鼠中进行口腔粘膜刺激试验。将颊囊外翻，使用发光二极管以275 mW/cm 2的辐照度用纯水、1 mg/mL咖啡酸或1 mg/mL葡萄籽提取物的UVA照射（波长：365 nm）处理。每次处理进行2或5分钟，重复三次。末次给药后24 h进行肉眼和组织学评价。我们还检查了治疗对大鼠皮肤伤口愈合过程的影响。如上所述处理在大鼠背部产生的全层皮肤伤口2分钟，但仅处理一次。然后每天评估伤口面积，持续9天。结果表明，治疗诱导口腔粘膜刺激依赖于照射时间;然而，它不影响伤口愈合过程。根据组织学分析，进行2分钟和5分钟的三次治疗的口腔粘膜刺激潜力分别为极轻微和轻度至中度。这些发现表明，考虑到抗菌作用的临床疗效和刺激潜力，基于口腔中多酚的UVA照射的治疗持续时间应尽可能短。
Antimicrobial chemotherapy based on ultraviolet-A (UVA) irradiation of polyphenol solution has been proposed as an adjunctive treatment for dental caries. However, the safety of this treatment has not been thoroughly evaluated. Therefore, the aim of the present study was to assess the influence of this treatment on the oral mucosa in hamsters and wounded skin in rats. An oral mucosal irritation test was performed in hamsters. The cheek pouch was everted and treated with UVA irradiation (wavelength: 365 nm) of pure water, 1 mg/mL of caffeic acid, or 1 mg/mL of grape seed extract using a light-emitting diode at an irradiance of 275 mW/cm2. Each treatment was performed for 2 or 5 min and repeated three times. Macroscopic and histological evaluations were performed 24 h after the last treatment. We also examined the effects of the treatment on the healing process of skin wounds in rats. Full-thickness skin wounds created on the back of the rats were treated for 2 min as described above, but only once. The wound area was then assessed daily for 9 days. The results demonstrated that the treatment induced oral mucosal irritation depending on the irradiation time; however, it did not influence the wound healing process. The oral mucosal irritation potential of three treatment sessions performed for 2 and 5 min was minimal and mild to moderate, respectively, according to histological analysis. These findings suggest that the duration of treatment based on UVA irradiation of polyphenols in the oral cavity should be as short as possible, considering the clinical efficacy of the antimicrobial effects and the irritation potential.