Differential susceptibility of Hyalomma excavatum adults and nymphs to the entomopathogens Metarhizium anisopliae ARSEF 4556 and Steinernema carpocapsae
Differential susceptibility of Hyalomma excavatum adults and nymphs to the entomopathogens Metarhizium anisopliae ARSEF 4556 and Steinernema carpocapsae
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DOI：
10.1080/09670874.2016.1181287
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发表时间：
2016-07-01
影响因子：
1.5
通讯作者：
Hazir, Selcuk
中科院分区：
文献类型：
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作者：
Butt, Tariq M.;Wood, Martyn;Hazir, Selcuk
本研究研究了昆虫病原真菌绿僵菌ARSEF 4556和昆虫病原线虫carpocapssteinerma carpocapsae对成虫和雌雄眼蜱的毒力。以10(6)、10(7)和10(8)分生孢子ml（-1）三种不同浓度对真菌进行检测。蜱虫暴露于0.5ml蒸馏水中4000ijs的carpocapsae，培养皿内衬2层湿滤纸。成蜱对绿僵菌不敏感，而若虫对绿僵菌敏感。若虫死亡率呈剂量依赖性，接种96小时后最高剂量杀死所有蜱虫。相比之下，carpocapae对饱食的成虫比未饱食的成虫或若虫更有效。蜱的易感性不受性别影响。未喂养的成年雄、雌、若虫的平均死亡率分别为30% +/- 9.2%、40% +/- 7.5%和36% +/- 4.7%。虫蛹死亡率为36%，与对照组比较差异有统计学意义（P < 0.05）。
This study investigated the virulence of the entomopathogenic fungus, Metarhizium anisopliae strain ARSEF 4556, and the entomopathogenic nematode Steinernema carpocapsae against adult and nymphal stages of the tick Hyalomma anatolicum excavatum. The fungus was tested at three different concentrations, 10(6), 10(7), and 10(8) conidia ml(-1). Ticks were exposed to 4000 IJs of S. carpocapsae in 0.5ml distilled water in Petri dishes lined with 2 layers of moist filter paper. Adult ticks were not susceptible to M. anisopliae, whereas the nymphs were highly susceptible. Nymph mortality was dose dependent with all ticks being killed at the highest dose 96hours post-inoculation. In contrast, S. carpocapsae was much more effective against engorged adults than unfed adults or nymphs. Tick susceptibility was not influenced by gender. The mean mortalities of the unfed adult males, females, and nymphs were 30% +/- 9.2%, 40% +/- 7.5%, and 36% +/- 4.7%, respectively. Nymphal mortality was 36% and there was a significant difference between nematode treatment and control group (P < 0.05).