Sorghum (Sorghum bicolor) Extract Affects Plasma Lipid Metabolism and Hepatic Macrophage Infiltration in Diabetic Rats
Sorghum (Sorghum bicolor) Extract Affects Plasma Lipid Metabolism and Hepatic Macrophage Infiltration in Diabetic Rats
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DOI：
10.2174/1573401315666190114153933
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发表时间：
2020-05
影响因子：
0.9
通讯作者：
Y. Mukai;S. Kataoka;Shin Sato
中科院分区：
文献类型：
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作者：
Y. Mukai;S. Kataoka;Shin Sato
慢性高血糖是进行性慢性肝病的高危因素，如脂质代谢异常。AMP激活的蛋白激酶（AMPK）的激活对血脂异常具有有益作用。来源于各种植物的多酚参与AMPK活化。我们研究了高粱多酚提取物（SE）对糖尿病大鼠血浆脂质代谢和巨噬细胞浸润的影响，并测定了AMPK和乙酰辅酶A羧化酶（ACC）的表达和磷酸化。链脲佐菌素诱导的糖尿病大鼠口服0、50或250 mg/kg SE 4周。检查血液化学、AMPK和ACC的总蛋白和磷酸化蛋白水平、固醇调节元件结合蛋白-1c（SREBP-1c）mRNA和蛋白水平以及肝脏中的巨噬细胞浸润。血糖和三酰甘油水平在未治疗的糖尿病大鼠中升高，但在250 mg/kg SE治疗的糖尿病大鼠中显著降低。与未治疗的大鼠相比，250 mg/kg SE治疗的糖尿病大鼠中AMPK和ACC磷酸化水平显著升高。糖尿病大鼠肝脏SREBP-1c的表达在各组间无差异。250 mg/kg SE处理抑制了肝脏中的巨噬细胞浸润。这些数据表明，SE治疗可能通过上调糖尿病大鼠肝脏AMPK和ACC的磷酸化来影响血脂代谢和慢性炎症。
Chronic hyperglycemia is known to be a high-risk factor for progressive chronic liver diseases, such as abnormal lipid metabolism. The activation of AMP-activated protein kinase (AMPK) has a beneficial effect on dyslipidemia. Polyphenols derived from various plants are involved in AMPK activation. We investigated the effects of polyphenol-containing sorghum (Sorghum bicolor) extract (SE) on plasma lipid metabolism and macrophage infiltration, and measured the expression and phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in diabetic rat livers. Streptozotocin-induced diabetic rats received 0, 50, or 250 mg/kg of SE orally for 4 weeks. Blood chemistry, total and phosphorylated protein levels of AMPK and ACC, sterol regulatory element- binding protein-1c (SREBP-1c) mRNA and protein levels, and macrophage infiltration in the livers were examined. Plasma glucose and triacylglycerol levels, which were increased in the untreated diabetic rats, were significantly lower in the 250 mg/kg SE-treated diabetic rats. AMPK and ACC phosphorylation levels were significantly increased in the 250 mg/kg SE-treated diabetic rats compared with those in the untreated rats. There was no difference in the hepatic expression of SREBP-1c between the diabetic rat groups. Macrophage infiltration in the liver was suppressed by 250 mg/kg of SEtreatment. These data suggest that SE treatment may affect plasma lipid metabolism and chronic inflammation by upregulating phosphorylation of AMPK and ACC in diabetic rat livers.