Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis.
Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis.
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DOI：
10.2147/ijn.s24160
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发表时间：
2011
影响因子：
8
通讯作者：
Ueoka R
中科院分区：
文献类型：
--
作者：
Komizu Y;Ueoka H;Goto K;Ueoka R
混合脂质体可以通过简单地超声处理缓冲溶液中的囊泡和胶束分子的混合物来制备。在这项研究中，我们研究了混合脂质体对体外人结肠癌细胞生长的影响。采用超声法制备了L-α-二肉豆蔻酰磷脂酰胆碱（DMPC）和聚氧乙烯（n）十二烷基醚（C12（EO）n，n = 21,23,25）混合脂质体（HL-n，n = 21,23,25），并在体外检测其对人结肠癌HCT116细胞生长的抑制作用。在 HL-n 存在的情况下观察到 HCT116 细胞的生长受到显着抑制。 HL-n 的百分之五十抑制浓度 (IC50) 低于 DMPC 脂质体的一半。此外，荧光显微镜和流式细胞术分析表明，HL-n对HCT116细胞生长的显着抑制作用可以通过诱导细胞周期停滞在G0/G1期以及细胞凋亡来实现。首次发现HL-n可以诱导结肠癌细胞的细胞周期停滞和细胞凋亡。这项研究的结果应有助于开发新的结肠癌化疗方案。
Hybrid liposomes can be prepared by simply sonicating a mixture of vesicular and micellar molecules in buffer solutions. In this study, we investigated the effects of hybrid liposomes on the growth of human colon cancer cells in vitro. Hybrid liposomes (HL-n, n = 21, 23, 25) composed of L-α-dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene(n) dodecyl ethers (C12(EO)n, n = 21, 23, 25) were prepared by the sonication method and their inhibitory effects on growth of human colon cancer HCT116 cells were examined in vitro. Significant growth inhibition of HCT116 cells was observed in the presence of HL-n. The fifty percent inhibitory concentration (IC50) of HL-n was less than half that of DMPC liposomes. Furthermore, fluorescence microscopic and flow cytometric analyses indicated that the markedly inhibitory effects of HL-n on the growth of HCT116 cells could be attained through the induction of cell cycle arrest at the G0/G1 phase along with apoptotic cell death. It was found for the first time that HL-n can induce both cell cycle arrest and apoptosis in colon cancer cells. The findings in this study should contribute to novel chemotherapy for colon cancer.