Progenitor Hyperpolarization Regulates the Sequential Generation of Neuronal Subtypes in the Developing Neocortex.
Progenitor Hyperpolarization Regulates the Sequential Generation of Neuronal Subtypes in the Developing Neocortex.
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DOI：
10.1016/j.cell.2018.06.036
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发表时间：
2018-08-23
期刊：
影响因子：
64.5
通讯作者：
Jabaudon D
中科院分区：
文献类型：
--
作者：
Vitali I;Fièvre S;Telley L;Oberst P;Bariselli S;Frangeul L;Baumann N;McMahon JJ;Klingler E;Bocchi R;Kiss JZ;Bellone C;Silver DL;Jabaudon D
在皮质发生过程中，脑室区祖细胞依次产生不同的神经元亚型，解释了新皮质细胞和它们形成的回路的多样性。虽然活性依赖性过程对于有丝分裂后神经元的分化和电路组装至关重要，但生物电过程如何影响非兴奋性细胞（如祖细胞）仍在很大程度上未知。在这里，我们揭示，在发育中的小鼠新皮层，脑室区祖细胞变得更加超极化，因为它们产生连续的神经元亚型。实验在体内超极化转移的转录程序和分裂模式，这些祖细胞到后来的发展状态，早熟一代的中间祖细胞和向前移动的层状，分子，形态和电路功能的神经元后代。这些作用是通过超极化抑制Wnt-β-连环蛋白信号通路而发生的。因此，在皮质生成过程中，生物电膜的特性允许特定的分子通路来协调祖细胞发育程序的时间进程，从而协调新皮质神经元的多样性。皮质发生过程中神经祖细胞膜电位的渐进性变化协调了新皮质神经元亚型规范的时间进程。
During corticogenesis, ventricular zone progenitors sequentially generate distinct subtypes of neurons, accounting for the diversity of neocortical cells and the circuits they form. While activity-dependent processes are critical for the differentiation and circuit assembly of postmitotic neurons, how bioelectrical processes affect non-excitable cells such as progenitors remains largely unknown. Here, we reveal that, in the developing mouse neocortex, ventricular zone progenitors become more hyperpolarized as they generate successive subtypes of neurons. Experimental in vivo hyperpolarization shifted the transcriptional programs and division modes of these progenitors to a later developmental status, with precocious generation of intermediate progenitors and a forward shift in the laminar, molecular, morphological and circuit features of their neuronal progeny. These effects occurred through inhibition of the Wnt-beta-catenin signaling pathway by hyperpolarization. Thus, during corticogenesis, bioelectric membrane properties are permissive for specific molecular pathways to coordinate the temporal progression of progenitor developmental programs and hence neocortical neuron diversity. Progressive changes in the membrane potential of neural progenitors during corticogenesis coordinates the temporal progression of neuronal subtype specification in neocortex.
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影响因子：
16.6
作者：
Bocchi R;Egervari K;Carol-Perdiguer L;Viale B;Quairiaux C;De Roo M;Boitard M;Oskouie S;Salmon P;Kiss JZ
通讯作者：
Kiss JZ
DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
DOI：
10.1242/dev.108043
发表时间：
2014-09
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Jacobshagen M;Niquille M;Chaumont-Dubel S;Marin P;Dayer A
通讯作者：
Dayer A
影响因子：
16.2
作者：
Ataman, Bulent;Ashley, James;Budnik, Vivian
通讯作者：
Budnik, Vivian
影响因子：
64.8
作者：
Iwasato, T;Datwani, A;Itohara, S
通讯作者：
Itohara, S