Effect of sterilization techniques prior to antimicrobial testing on physical properties of dental restorative materials
Effect of sterilization techniques prior to antimicrobial testing on physical properties of dental restorative materials
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DOI：
10.1016/j.jdent.2015.03.012
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发表时间：
2015-06-01
影响因子：
4.4
通讯作者：
Camilleri, Josette
中科院分区：
文献类型：
--
作者：
Farrugia, Cher;Cassar, Glenn;Camilleri, Josette
目的：本研究的目的是调查所选牙科材料在进行抗菌测试之前进行灭菌后微观结构和表面性能的任何变化。方法：研究的材料包括牙本质替代材料Chemfil上级（R）、Ionoseal（R）、Dyract Extra（R）和SDR（R）。用扫描电子显微镜（SEM）和能谱仪（EDS）对材料进行了表征。使用酒精、蒸汽、紫外线（UV）和环氧乙烷对试验材料进行灭菌，然后通过SEM、显微硬度试验和傅里叶变换红外（FT-IR）光谱法评估这些材料的任何变化。通过平板计数技术和浊度试验评估处理前的材料微生物水平。通过分析CFU/sample.Results可能的污染通过分配器进行评估：环氧乙烷影响的Chemfil，Ionoseal和Dyract的微观结构，导致平坦的Si-O伸缩振动和沉积的氯和钙分别在Chemfil和Dyract。不同的污染被证明对所有材料在厌氧conditions.Conclusions培养时：不同的灭菌技术影响下调查的材料的微观结构。在无菌条件下生产的材料样品也可能被细菌污染，无论是来自材料本身还是通过分配设备。临床意义：抗菌研究的结果不能在临床上推断，因为材料灭菌处理导致材料化学和微观结构的变化，这反过来可能影响材料的抗菌活性。(C)2015爱思唯尔有限公司版权所有。
Objectives: The aim of this study was to investigate any changes to the microstructure and surface properties of selected dental materials after sterilization carried out prior to subjecting them to antimicrobial testing. Initial microbial contamination on the material, as well as other possible sources of contamination were also assessed.Methods: The materials investigated included dentine replacement materials Chemfil Superior(R), Ionoseal(R), Dyract Extra(R) and SDR(R). The materials were characterized by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The test materials were sterilized using alcohol, steam, ultraviolet light (UV) and ethylene oxide and any changes to these materials were then assessed by SEM, microhardness testing and Fourier transform infrared (FT-IR) spectroscopy. Material microbial levels before treatments were assessed by plate counting technique and turbidity tests. Possible contamination through dispensers was assessed by analysing the CFU/sample.Results: Ethylene oxide affected the microstructure of the Chemfil, Ionoseal and Dyract, resulting in flattening of the Si-O stretching vibrations and deposition of chlorine and calcium respectively in Chemfil and Dyract. Varied contamination was demonstrated on all materials when incubated in anaerobic conditions.Conclusions: The different sterilization techniques affected the microstructure of the materials under investigation. Samples of materials produced in sterile conditions could also be contaminated with bacteria, either from the material itself or through the dispensing apparatus.Clinical significance: Results of antimicrobial studies cannot be extrapolated clinically as the material sterilization treatment results in changes to material chemistry and microstructure, which could in turn affect the materials' antimicrobial activity. (C) 2015 Elsevier Ltd. All rights reserved.