SURFACE-MODIFIED POLY(METHYL METHACRYLATE) ENHANCES ADSORPTION AND RETAINS ANTICANDIDAL ACTIVITIES OF SALIVARY HISTATIN-5
SURFACE-MODIFIED POLY(METHYL METHACRYLATE) ENHANCES ADSORPTION AND RETAINS ANTICANDIDAL ACTIVITIES OF SALIVARY HISTATIN-5
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DOI：
10.1002/jbm.820291015
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发表时间：
1995-10-01
期刊：
影响因子：
--
通讯作者：
LEVINE, MJ
中科院分区：
文献类型：
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作者：
EDGERTON, M;RAJ, PA;LEVINE, MJ
义齿引起的口腔炎是一种常见的口腔内疾病，与义齿表面高水平的白色念珠菌粘附有关。本研究的目的是生产一种表面改性的义齿树脂，该树脂通常由聚甲基丙烯酸甲酯 (PMMA) 制成，带有固定化抗念珠菌蛋白。通过甲基丙烯酸甲酯的表面聚合对 PMMA 进行改性，以增强对有效的念珠菌唾液蛋白组蛋白 5 的吸附。与未改性的材料相比，改性的 PMMA 对组蛋白 5 表现出更高的表面吸附和解吸。由于组蛋白 5 会破坏白色念珠菌细胞膜的稳定性并允许细胞内分子流出，因此通过真菌细胞的染料释放来监测念珠菌活性。吸附的组蛋白 5 不会从酵母细胞中释放染料；然而，当组蛋白从表面解吸时，检测到染料。在粘附测定中，改性 PMMA 降低了人颌下舌下唾液 (HSMSL) 介导的酵母细胞与聚合物的粘附。将组蛋白 5 预涂到未修饰的 PMMA 上也消除了 HMSSL 介导的粘附。这些实验表明，牙科用丙烯酸树脂可以进行表面改性并负载组蛋白 5，作为控制组蛋白 5 释放到受影响区域的手段。这种表面修饰还可以减少白色念珠菌细胞对唾液涂覆材料的粘附。 (C) 1995 约翰威利父子公司
Denture-induced stomatitis is a common intraoral disease which is associated with high levels of Candida albicans adhesion to a denture surface. The aim of this study was to produce a surface-modified denture resin, which is usually manufactured from poly(methyl methacrylate) (PMMA), carrying an immobilized anticandidal protein. PMMA was modified by surface polymerization of methyl methacrylic acid to enhance adsorption of a potent candidacidal salivary protein, histatin 5. The modified PMMA showed higher surface adsorption and desorption of histatin 5 than the unmodified material. Because histatin 5 destabilizes C. albicans cell membranes and allows efflux of intracellular molecules, candidacidal activity was monitored by dye release from fungal cells. Adsorbed histatin 5 did not release dye from the yeast cells; however, dye was detected as histatin was desorbed from the surface. In an adhesion assay, modified PMMA decreased human submandibular-sublingual saliva (HSMSL) mediated adherence of yeast cells to the polymer. Precoating histatin 5 onto unmodified PMMA also abolished HSMSL-mediated adhesion. These experiments show that dental acrylic may be surface modified and loaded with histatin 5 as a means of controlled release of histatin 5 to an affected area. This surface modification may additionally reduce adhesion of C. albicans cells to the saliva-coated material. (C) 1995 John Wiley & Sons, Inc.