Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo
Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo
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DOI：
10.4049/jimmunol.166.10.6099
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发表时间：
2001-05-15
影响因子：
4.4
通讯作者：
Heath, WR
中科院分区：
文献类型：
--
作者：
Li, M;Davey, GM;Heath, WR
为了更好地了解交叉呈递的抗原要求，我们比较了细胞相关 OVA 与可溶性 OVA 与 OT-I (CD8) 和 OT-II (CD4) TCR 转基因系的体内呈递效率。细胞相关 OVA 的交叉呈递非常有效，只需 21 ng OVA 即可激活 OT-II 细胞，而激活 OT-I 细胞则少 100 倍。相比之下，可溶性 OVA 的呈递效率较低，需要至少 10,000 ng OVA 才能激活任一 T 细胞亚群。因此，细胞相关的 OVA 呈递给 CD4 T 细胞的效率比可溶性 OVA 的效率高 500 倍，呈递给 CD8 T 细胞的效率高 50,000 倍。这些数据代表了交叉呈递的首次体内定量分析，表明细胞相关的 OVA 通过 1 类途径非常有效地呈递。
To better understand the antigenic requirements for cross-presentation, we compared the in vivo efficiency of presentation of cell-associated vs soluble OVA with the OT-I (CD8) and OT-II (CD4) TCR transgenic lines. Cross-presentation of cell-associated OVA was very efficient, requiring as little as 21 ng of OVA to activate OT-II cells and 100-fold less to activate OT-I cells. In contrast, soluble OVA was presented inefficiently, requiring at least 10,000 ng OVA for activation of either T cell subset. Thus, cell-associated OVA was presented 500-fold more efficiently than soluble OVA to CD4 T cells and 50,000-fold more efficiently to CD8 T cells. These data, which represent the first quantitative in vivo analysis of cross-presentation, show that cell-associated OVA is very efficiently presented via the class 1 pathway.