PLASMIDS OF THE SAME INC GROUPS IN ENTEROBACTERIA BEFORE AND AFTER THE MEDICAL USE OF ANTIBIOTICS
PLASMIDS OF THE SAME INC GROUPS IN ENTEROBACTERIA BEFORE AND AFTER THE MEDICAL USE OF ANTIBIOTICS
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DOI：
10.1038/306616a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
HUGHES, VM
中科院分区：
文献类型：
--
作者：
DATTA, N;HUGHES, VM
接合质粒在医学使用抗生素之前分离的肠细菌中很常见。大肠杆菌 K-12 的质粒 F 就是一个例子，我们在 1917 年至 1954 年间分离的菌株集合（默里菌株集合）中超过 20% 中鉴定了其他质粒。在过去的 25 年里，编码抗生素抗性的接合质粒在与 Murray Collection 相同属的细菌中变得很常见——沙门氏菌、志贺氏菌、克雷伯氏菌、变形杆菌、埃希氏菌。本研究旨在证明“抗生素前”质粒是否与现代 R 质粒属于同一组（通过不相容性测试（Inc 组）定义）。在大肠杆菌中建立了 84 个这样的质粒。 coliK-12，不具有抗生素抗性决定簇，65 个与现有抗性 (R) 质粒属于同一组。因此，在过去 25 年中，医学上重要的细菌获得抗生素抗性的显着方式似乎是通过将新基因插入现有质粒，而不是通过以前罕见的质粒的传播。
Conjugative plasmids were common in enterobacteria isolated before the medical use of antibiotics. Plasmid F ofEscherichia coliK-12 was one example and we1identified others in over 20% of a collection of strains isolated between 1917 and 1954, the Murray collection. In the past 25 years, conjugative plasmids encoding antibiotic resistances have become common in bacteria of the same genera as those of the Murray Collection—Salmonella, Shigella, Klebsiella, Proteus, Escherichia. The present study was made to show whether the ‘pre-antibiotic’ plasmids belonged to the same groups, as defined by incompatibility tests (Inc groups), as modern R plasmids. Of 84 such plasmids established inE. coliK-12, none with antibiotic resistance determinants, 65 belonged to the same groups as present resistance (R) plasmids. Thus the remarkable way in which medically important bacteria have acquired antibiotic resistance in the past 25 years seems to have been by the insertion of new genes into existing plasmids rather than by the spread of previously rare plasmids.