ISOLATION OF RENIN-RICH RAT-KIDNEY CELLS
ISOLATION OF RENIN-RICH RAT-KIDNEY CELLS
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DOI：
10.1161/01.hyp.10.5.488
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发表时间：
1987-11-01
期刊：
影响因子：
8.3
通讯作者：
PEACH, MJ
中科院分区：
文献类型：
--
作者：
JOHNS, DW;CAREY, RM;PEACH, MJ
酶分散和密度梯度（Percoll）沉降被用来从大鼠肾皮质分离一群含肾素的颗粒负载细胞（密度1.067 g/ml）。使用免疫组织化学（光学显微镜）和电子显微镜，我们确定了这些细胞的存在和储存肾素蛋白的能力。使用1000个碱基对的大鼠肾素互补DNA来显示这些细胞表达肾素基因。反向溶血空斑试验确定了含肾素细胞的功能特性。这些数据与钙浓度和肾素释放之间的假定反比关系是一致的。因此，我们已经分离出一群功能性大鼠肾细胞，合成，储存和释放肾素。
Enzymatic dispersion and density gradient (Percoll) sedimentation were used to isolate a population of renin-containing, granule-laden cells (density 1.067 g/ml) from rat kidney cortex. Using immunohistochemistry (light microscopy) and electron microscopy, we defined the presence and ability of these cells to store renin protein(s). A 1000 base pair rat renin complementary DNA was used to show that these cells express the renin gene. The reverse hemolytic plaque assay defined the functional properties of the renin-containing cell. The data are consistent with the postulated inverse relationship between calcium concentration and release of renin. Thus, we have isolated a population of functional rat kidney cells that synthesize, store, and release renin.