Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot.
Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot.
复制标题
哈茨木霉对玉米根际微生物群的影响及镰刀菌茎腐病的生防
DOI：
10.1038/s41598-017-01680-w
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发表时间：
2017-05-11
影响因子：
4.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Saravanakumar K;Li Y;Yu C;Wang QQ;Wang M;Sun J;Gao JX;Chen J
由禾谷镰刀菌（Fusarium graminearum，FG）引起的镰刀菌茎腐病（Fusarium stalk rot，FSR）严重影响玉米的产量。使用农药防治该病对环境有一定的危害。在这方面，使用生物防治剂（BCA）是农业化学品的替代品。虽然木霉属物种被称为BCA，但宿主-病原体特异性木霉的选择对于成功的田间应用至关重要。因此，本研究从100株木霉菌中筛选出抗玉米根际真菌病的木霉菌株，并选择哈茨木霉菌（Trichoderma harzianum）（CCTCC-RW 0024）进行温室试验，研究其对玉米根际微生物组变化及FSR生物防治的影响。菌株CCTCC-RW 0024具有较高的拮抗活性（96.30%）、防病效果（86.66%）、生防相关酶活性和基因表达量。通过eGFP标记和qRT-PCR分析证实菌株的根定殖。焦磷酸测序结果表明，外源接种该菌株后，玉米根际促生酸杆菌数量增加了18.4%，FG减少了66%，同时也促进了玉米的生长。此外，该菌株的代谢产物可以与致病性相关的转录辅因子FgSW 16相互作用，从而有助于其抑制。结论是T.哈茨菌株CCTCC-RW 0024是一株具有抗FSR潜力的BCA。
Fusarium stalk rot (FSR) caused by Fusarium graminearum (FG) significantly affects the productivity of maize grain crops. Application of agrochemicals to control the disease is harmful to environment. In this regard, use of biocontrol agent (BCA) is an alternative to agrochemicals. Although Trichoderma species are known as BCA, the selection of host-pathogen specific Trichoderma is essential for the successful field application. Hence, we screened a total of 100 Trichoderma isolates against FG, selected Trichoderma harzianum (CCTCC-RW0024) for greenhouse experiments and studied its effect on changes of maize rhizosphere microbiome and biocontrol of FSR. The strain CCTCC-RW0024 displayed high antagonistic activity (96.30%), disease reduction (86.66%), biocontrol-related enzyme and gene expression. The root colonization of the strain was confirmed by eGFP tagging and qRT-PCR analysis. Pyrosequencing revealed that exogenous inoculation of the strain in maize rhizosphere increased the plant growth promoting acidobacteria (18.4%), decreased 66% of FG, and also increased the plant growth. In addition, metabolites of this strain could interact with pathogenicity related transcriptional cofactor FgSWi6, thereby contributing to its inhibition. It is concluded that T. harzianum strain CCTCC-RW0024 is a potential BCA against FSR.