The canonical Wnt pathway directly regulates NRSF/REST expression in chick spinal cord
The canonical Wnt pathway directly regulates NRSF/REST expression in chick spinal cord
复制标题
DOI：
10.1016/j.bbrc.2003.09.158
复制
发表时间：
2003-11-07
影响因子：
3.1
通讯作者：
Ogura, T
中科院分区：
文献类型：
--
作者：
Nishihara, S;Tsuda, L;Ogura, T
神经前体细胞在心室区中活跃增殖以自我更新干细胞群体，并且平行地维持其未分化状态。该祖细胞库产生有丝分裂后的细胞，其迁移到套层并分化成成熟的神经元。这些干细胞的生长受到经典Wnt信号级联的严格控制，部分由细胞周期蛋白D1的直接调节介导，细胞周期蛋白D1是细胞周期进程的关键调节因子。在这里，我们报告，经典的Wnt通路直接控制NRSF/REST的表达。Wnt激活的β-连环蛋白/TCF复合物通过在其外显子1a中发现的保守元件上调该基因，这是通过一种新的卵内转录测定获得的关键结果。因此，我们的数据表明，经典的Wnt信号级联直接调节NRSF/REST和细胞周期蛋白D1基因，从而控制祖细胞。此外，我们表明，我们的卵内转录试验是一种强大的方式来分析基因调控在一个自然的体内环境。(C)2003年爱思唯尔公司All rights reserved.
Neural precursor cells actively proliferate in the ventricular zone to self-renew the stem cell population, and in parallel, maintain their undifferentiated state. This progenitor pool generates postmitotic cells that migrate to the mantle layer and differentiate into mature neurons. The growth of these stem cells is strictly controlled by the canonical Wnt signaling cascade, in part mediated by the direct regulation of Cyclin D1, a critical regulator of cell cycle progression. Here, we report that the canonical Wnt pathway directly controls the expression of NRSF/REST. The Wnt-activated beta-catenin/TCF complex up-regulates this gene through a conserved element found in its exon 1a, a critical result obtained by a novel in ovo transcriptional assay. Hence, our data show that the canonical Wnt signaling cascade directly regulates the NRSF/REST and Cyclin D1 genes, thereby controlling the progenitor cells. In addition, we show that our in ovo transcription assay is a powerful way to analyze gene regulation in a natural in vivo context. (C) 2003 Elsevier Inc. All rights reserved.