A comparative long-term operation using up-flow anaerobic sludge blanket (UASB) and anaerobic membrane bioreactor (AnMBR) for the upgrading of anaerobic treatment of N, N-dimethylformamide-containing wastewater
A comparative long-term operation using up-flow anaerobic sludge blanket (UASB) and anaerobic membrane bioreactor (AnMBR) for the upgrading of anaerobic treatment of N, N-dimethylformamide-containing wastewater
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DOI：
10.1016/j.scitotenv.2019.134370
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发表时间：
2020-01-10
影响因子：
9.8
通讯作者：
Li, Yu-You
中科院分区：
文献类型：
--
作者：
Li, Lu;Kong, Zhe;Li, Yu-You
采用厌氧污泥床（UASB）和厌氧膜生物反应器（AnMBR）处理含N，N-二甲基甲酰胺（DMF）约2000 mg/L的人工合成工业废水。接种物由两种污泥来源组成：AnMBR中厌氧消化污泥（ADS）与DMF水解活性污泥（DAS）的共培养物，UASB中厌氧颗粒污泥（AGS）与DAS的共培养物。在第一天，在两个反应器中实现了有效的DMF产甲烷降解，去除率接近100%。两种反应器在3-4 g COD/L/d的低有机负荷下均获得了优异的DMF去除效率和高的甲烷产量。然而，OLR的过度升高显著限制DMF水解。当OLR超过6 g COD/L/d时，两种反应器的去除效率和甲烷产量均急剧下降。尽管它们的形式和形状不同，但ADS和AGS都提供负责产甲烷的产甲烷菌。UASB耐受较高的OLR，而AnMBR由于污泥浓度增加而受到膜污染的限制。然而，AnMBR获得了高质量的出水，没有悬浮固体。DMF能否被有效降解取决于DAS，其中丰富的DMF水解细菌（DHB）为DMF的有效水解提供了足够数量的水解酶。然而，这些DHB是兼性的，也被鉴定为需要硝酸盐作为电子受体或在有氧条件下存活的微生物。它们逐渐腐烂而不是增殖，并被产甲烷菌击败。因此，可以想象的是，少量的硝酸盐将在UASB和AnMBR中富集DHB的丰度，并为DMF水解提供足够量的酶。利用UASB培养厌氧DMF降解颗粒污泥被认为是有效处理含DMF废水的升级解决方案。(C)2019 Elsevier B. V.版权所有。
Synthetic industrial wastewater containing approximately 2000 mg/L N, N-dimethylformamide (DMF) was treated using a lab-scale anaerobic sludge blanket (UASB) and an anaerobic membrane bioreactor (AnMBR) in this study. The inoculum consisted of two sources of sludge: Co-culture of anaerobic digested sludge (ADS) with DMF-hydrolyzing activated sludge (DAS) for the AnMBR, and co-culture of anaerobic granular sludge (AGS) with DAS for the UASB. Effective DMF methanogenic degradation of nearly 100% removal was achieved in both reactors on the first day. Both reactors obtained excellent DMF removal efficiency and high methane production under a low organic loading rate (OLR) of around 3-4 g COD/L/d. However, excessive elevation of OLR significantly limited DMF hydrolysis. When OLR exceeded 6 g COD/L/d, the removal efficiency and methane production in both reactors dramatically dropped. Despite their different forms and shapes, the ADS and AGS both provide methanogens which are responsible for methanogenesis. The UASB tolerated a higher OLR while the AnMBR was limited by membrane fouling due to the increased sludge concentration. However, the AnMBR obtained high-quality effluent without suspended solid. Whether DMF can be effectively degraded depends on DAS, in which abundant DMF-hydrolyzing bacteria (DHB) provide sufficient quantities of the hydrolytic enzyme for effective hydrolysis of DMF. However, these DHB were facultative and were also identified as denitrifying bacteria which require nitrate as the electron acceptor or otherwise survive under the aerobic condition. They gradually decayed rather than proliferated and were outcompeted by methanogens. Therefore, it is conceivable that a slight dosage of nitrate would enrich the abundance of DHB in both the UASB and the AnMBR, and provide a sufficient quantity of enzymes for the DMF hydrolysis. The cultivation of the anaerobic DMF-degrading granular sludge using the UASB is considered an upgraded solution to the effective treatment of DMF-containing wastewater. (C) 2019 Elsevier B.V. All rights reserved.