Aptamer based photometric assay for the antibiotic sulfadimethoxine based on the inhibition and reactivation of the peroxidase-like activity of gold nanoparticles
Aptamer based photometric assay for the antibiotic sulfadimethoxine based on the inhibition and reactivation of the peroxidase-like activity of gold nanoparticles
复制标题
DOI：
10.1007/s00604-016-1994-1
复制
发表时间：
2016
影响因子：
5.7
通讯作者：
Jiao Yan;Yafei Huang;Chenghui Zhang;Z. Fang;Wenhui Bai;Mengmeng Yan;Chao Zhu;Ailiang Chen
中科院分区：
文献类型：
--
作者：
Jiao Yan;Yafei Huang;Chenghui Zhang;Z. Fang;Wenhui Bai;Mengmeng Yan;Chao Zhu;Ailiang Chen
众所周知，金纳米颗粒（AuNP）具有类似过氧化物酶的活性。它们可以催化 H2O2 氧化 3,3,5,5-四甲基联苯胺，导致颜色从红色变为蓝色。这里表明，AuNPs 的过氧化物酶样活性可以通过用针对磺胺二甲氧嘧啶的 ssDNA 适配体钝化其表面钝化来抑制。然而，如果目标分子（磺胺二甲氧嘧啶）存在，适体就会从 AuNPs 表面解吸，从而导致 AuNPs 催化性能的重新激活。溶液的颜色变化（从紫色到蓝色）与分析物浓度有关，这可以通过目视或通过 650 nm 处的紫外可见吸收光度法来判断。在优化条件下，该测定的磺胺二甲氧嘧啶检测限为 10 ng·mL−1，并且校准图在相当宽的浓度范围（0.01–1000 μg·mL−1）内呈线性。该测定可在 15 分钟内完成，并且非常灵敏，因此非常适合食品分析中的快速筛选。可以想象，它可以扩展到许多其他可用适配体的小分析物。图解摘要基于金纳米粒子 (AuNP) 过氧化物酶样活性的抑制和再激活的磺胺二甲氧嘧啶 (SDM) 光度测定具有相当宽的线性范围 (0.01–1000 µg•mL−1) 和 10 ng•mL−1 的低检测限。
It is known that gold nanoparticles (AuNPs) possess peroxidase-like activity. They can catalyze the oxidation of 3,3,5,5-tetramethylbenzidine by H2O2which leads to a color change from red to blue. It is shown here that the peroxidase-like activity of AuNPs can be inhibited by passivating its surface passivation with a ssDNA aptamer against sulfadimethoxine. If, however, the target molecule (sulfadimethoxine) is present, the aptamer is desorbed from the AuNPs surface, and this results in the reactivation of the catalytic property of the AuNPs. The color change of the solution (from purple to blue) is related to the analyte concentration, and this can be judged visually or by UV-visible absorptiometry at 650 nm. The assay, under optimized conditions, has a detection limit of 10 ng·mL−1of sulfadimethoxine, and the calibration plot is linear over a rather wide concentration range (0.01–1000 μg·mL−1). The assay can be performed within <15 min, is sensitive, and therefore is well suited for fast screening in food analysis. Conceivably, it can be extended to many other small analytes for which aptamers are available.Graphical abstractAptamer based photometric assay for sulfadimethoxine(SDM) based on the inhibition and reactivation of the peroxidase-like activity of gold nanoparticles (AuNPs) was performed with a rather wide linear range (0.01–1000 μg•mL−1) and low detection limit of 10 ng•mL−1.