Downregulation of interferon-γ-induced protein 10 in the tears of patients with Stevens-Johnson syndrome with severe ocular complications in the chronic stage.
Downregulation of interferon-γ-induced protein 10 in the tears of patients with Stevens-Johnson syndrome with severe ocular complications in the chronic stage.
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DOI：
10.1136/bmjophth-2017-000073
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发表时间：
2017
影响因子：
2.4
通讯作者：
Kinoshita S
中科院分区：
文献类型：
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作者：
Ueta M;Nishigaki H;Sotozono C;Kinoshita S
史蒂文斯-约翰逊综合征（SJS）和中毒性表皮坏死松解症（TEN）是皮肤和粘膜（例如眼表、口腔和生殖器）的急性炎症性囊泡性反应。一些皮肤科医生诊断的SJS/TEN患者会出现严重的眼部并发症（SOC）。为了研究SJS/TEN慢性期SOC眼表炎症的病理生理学，我们检测了眼表疾病患者和健康对照者泪液中的细胞因子。慢性期SJS/TEN眼（n>30）、健康眼（n>20，对照）和来自代表不同眼表面炎性病症的特应性角膜结膜炎患者的眼（n>20）。泪液样本收集在Schirmer测量条上。为了测量泪液中各种细胞因子的水平，我们使用BD CBA Flex套件。观察性研究（病例对照研究）。检测白细胞介素（IL）-6、IL-8、嗜酸性粒细胞趋化因子（eotaxin）、巨噬细胞炎性蛋白（MIP）-1β、RANTES（调节活化的正常T细胞表达和分泌）、干扰素γ（IFN）-γ、单核细胞趋化蛋白-1、IFN-γ诱导蛋白-10（IP-10）和总IgE水平。结果发现，与对照组相比，SJS/TEN伴SOC组IL-6、IL-8、eotaxin和MIP-1β表达显著上调，IP-10表达显著下调。与特应性角膜结膜炎相比，IP-10在SJS/TEN伴SOC中显著下调;另一方面，与SJS/TEN伴SOC相比，总IgE在特应性角膜结膜炎中显著上调。泪液中的IP-10可能是区分慢性SJS/TEN伴SOC和其他眼部炎症性疾病（如特应性角膜结膜炎）的生物标志物。
Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are acute inflammatory vesiculobullous reactions of the skin and mucosa such as the ocular surface, oral cavity and genitals. Severe ocular complications (SOC) arise in some patients with SJS/TEN diagnosed by dermatologists. To investigate the pathophysiology of ocular surface inflammation in SJS/TEN with SOC in the chronic stage, we examined cytokines in the tears of patients with ocular surface diseases and healthy controls. SJS/TEN eyes in the chronic stage (n>30), healthy eyes (n>20, controls) and eyes (n>20) from patients with atopic keratoconjunctivitis representing different ocular surface inflammatory disorders. Tear samples were collected on Schirmer's measurement strips. To measure the level of various cytokines in the tears we used BD CBA Flex sets. An observational study (case–control study). We recorded the level of interleukin (IL)-6, IL-8, eotaxin, macrophage inflammatory protein (MIP)-1β, RANTES (regulated on activation, normal T cell expressed and secreted), interferon gamma (IFN)-γ, monocyte chemoattractant protein-1, IFN-γ-induced protein 10 (IP-10) and total IgE. We found that compared with the controls, in SJS/TEN with SOC, IL-6, IL-8, eotaxin and MIP-1β were significantly upregulated while IP-10 was significantly downregulated. Compared with atopic keratoconjunctivitis, IP-10 was significantly downregulated in SJS/TEN with SOC; on the other hand, total IgE was significantly upregulated in atopic keratoconjunctivitis compared with SJS/TEN with SOC. IP-10 in tears may be a biomarker to distinguish between chronic SJS/TEN with SOC and other ocular inflammatory disorders such as atopic keratoconjunctivitis.