Prereperfusion saline infusion into ischemic territory reduces inflammatory injury after transient middle cerebral artery occlusion in rats
Prereperfusion saline infusion into ischemic territory reduces inflammatory injury after transient middle cerebral artery occlusion in rats
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DOI：
10.1161/01.str.0000028237.15541.cc
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发表时间：
2002-10-01
期刊：
影响因子：
8.3
通讯作者：
Diaz, FG
中科院分区：
文献类型：
--
作者：
Ding, YC;Li, J;Diaz, FG
背景和目的：在缺血性卒中中，缺血危机激活了一系列事件，这些事件被再灌注增强，最终导致细胞死亡。本研究的主要目的是研究我们新的脑卒中治疗实验模型，即在再灌注前用生理盐水冲洗缺血区域，是否可以通过(1)减少炎症反应和(2)改善局部微循环来减少这种损伤。方法：采用新型腔内空心灯丝封堵大脑中动脉2小时诱导sd大鼠（n=39）脑卒中。再灌注48小时前，20只缺血大鼠分别在23℃、37℃等渗生理盐水7 mL经纤维注入缺血区。用激光多普勒血流仪测定缺血和再灌注时大脑皮层由右大脑中动脉供血的区域血流。用苏木精和伊红染色比较白细胞浸润、微血管堵塞和梗死体积。免疫细胞化学检测细胞间粘附分子1 （ICAM-1）的表达。评估神经功能缺损。结果：经生理盐水预灌注后，脑缺血再灌注后，脑缺血再灌注后，脑缺血再灌注显著(P
Background and Purpose-In ischemic stroke, the ischemic crisis activates a cascade of events that are potentiated by reperfusion, eventually leading to cell death. The chief aim in this study was to investigate whether our new experimental model for stroke therapy, flushing the ischemic territory with saline before reperfusion, could minimize this damage by (1) reducing the inflammatory reaction and (2) improving regional microcirculation.Methods-Stroke in Sprague-Dawley rats (n=39) was induced by a 2-hour middle cerebral artery occlusion with the use of a novel intraluminal hollow filament. Before 48-hour reperfusion, 20 of the ischemic rats received 7 mL isotonic saline at 23degreesC or 37degreesC infused into the ischemic area through the filament. Regional cerebral blood flow in cortex supplied by the right middle cerebral artery was measured by laser-Doppler flowmetry during ischemia and reperfusion. Leukocyte infiltration, microvascular plugging, and infarct volume were compared with the use of hematoxylin and eosin staining. Expression of intercellular adhesion molecule 1 (ICAM-1) was determined by immunocytochemistry. Neurological deficits were evaluated.Results-After the prereperfusion infusion of saline, significantly (P