Improved liver function in patients with liver cirrhosis after autologous bone marrow cell infusion therapy
Improved liver function in patients with liver cirrhosis after autologous bone marrow cell infusion therapy
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DOI：
10.1634/stemcells.2005-0542
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发表时间：
2006-10-01
期刊：
影响因子：
5.2
通讯作者：
Sakaida, Isao
中科院分区：
文献类型：
--
作者：
Terai, Shuji;Ishikawa, Tsuyoshi;Sakaida, Isao
我们在此报告9例肝硬化（LC）患者接受外周静脉自体骨髓细胞输注（ABMI）。研究对象为总胆红素小于3.0 mg/dl、血小板计数大于5 （10(10)/l）、诊断影像学无活肝细胞癌的LC患者。全身麻醉下从髂骨中分离自体骨髓（BM, 400 ml）。细胞洗涤分离单核细胞，外周静脉输注。通过荧光活化细胞分选分析（CD34、CD45和c-kit）证实了MNC的特征。ABMI治疗后，通过血液检查监测肝功能24周。从400 ml的BM中，我们获得了7.81 +/- 0.98 x 10(9)个MNCs。洗净后，将5.20 +/- 0.63 × 10(9) MNCs输注到LC患者体内。血清白蛋白水平和总蛋白在ABMI治疗后24周显著改善(p
We here report nine liver cirrhosis (LC) patients that underwent autologous bone marrow cell infusion (ABMI) from the peripheral vein. Subjects were patients with LC with total bilirubin of less than 3.0 mg/dl, platelet count of more than 5 (10(10)/l), and no viable hepatocellular carcinoma on diagnostic imaging. Autologous bone marrow (BM; 400 ml) was isolated from the ilium under general anesthesia. Mononuclear cells (MNCs) were separated by cell washing and were infused via the peripheral vein. MNC characteristics were confirmed by fluorescence-activated cell sorting analysis (CD34, CD45, and c-kit). After ABMI therapy, liver function was monitored by blood examination for 24 weeks. From 400 ml of BM, we obtained 7.81 +/- 0.98 x 10(9) MNCs. After washing, 5.20 +/- 0.63 x 10(9) MNCs were infused into patients with LC. Significant improvements in serum albumin levels and total protein were observed at 24 weeks after ABMI therapy (p