Conservation of the abscission signaling peptide IDA during Angiosperm evolution: withstanding genome duplications and gain and loss of the receptors HAE/HSL2.
Conservation of the abscission signaling peptide IDA during Angiosperm evolution: withstanding genome duplications and gain and loss of the receptors HAE/HSL2.
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DOI：
10.3389/fpls.2015.00931
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发表时间：
2015
影响因子：
5.6
通讯作者：
Aalen RB
中科院分区：
文献类型：
--
作者：
Stø IM;Orr RJ;Fooyontphanich K;Jin X;Knutsen JM;Fischer U;Tranbarger TJ;Nordal I;Aalen RB
肽INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)通过富含亮氨酸的重复受体样激酶HAESA (HAE)和HAESA-LIKE2 (HSL2)发出信号，控制拟南芥中的不同细胞分离事件。我们假设该信号模块参与其他植物物种的脱落过程，即使它们可能脱落拟南芥以外的其他器官。作为从进化角度检验这一假设的第一步，我们通过对公开的被子植物蛋白质、核苷酸和基因组数据库进行 BLAST 搜索，确定了编码 IDA 及其受体推定直向同源物的基因。在所有研究的物种中都发现了编码 IDA 或 IDA-LIKE (IDL) 肽和 HSL 蛋白的基因，这些物种被选择来代表具有可用基因组序列的每个被子植物目。代表拟南芥生物活性肽的 12 个氨基酸在被子植物的整个进化过程中几乎没有变化；然而，IDL 和 HSL 基因的数量因目和物种而异。系统发育分析表明，IDA、HSL2 和相关的 HSL1 基因存在于产生被子植物的物种中。 HAE 是在单子叶植物-真双子叶植物分裂后发生基因组复制后由 HSL1 产生的。 HSL1 也在单子叶植物中独立复制，而 HSL2 在生姜 (Zingiberales) 和草 (Poales) 中丢失。 IDA 已在真双子叶植物中复制，产生功能不同的 IDL 肽。我们假设核心真双子叶植物中存在大量 IDL 同源物是多个全基因组重复 (WGD) 的结果。我们通过提供来自不同物种的不同器官分离事件的基因表达数据，证实了 IDA 和 HAE/HSL2 同源物在分离中的参与。
The peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), which signals through the leucine-rich repeat receptor-like kinases HAESA (HAE) and HAESA-LIKE2 (HSL2), controls different cell separation events in Arabidopsis thaliana. We hypothesize the involvement of this signaling module in abscission processes in other plant species even though they may shed other organs than A. thaliana. As the first step toward testing this hypothesis from an evolutionarily perspective we have identified genes encoding putative orthologs of IDA and its receptors by BLAST searches of publically available protein, nucleotide and genome databases for angiosperms. Genes encoding IDA or IDA-LIKE (IDL) peptides and HSL proteins were found in all investigated species, which were selected as to represent each angiosperm order with available genomic sequences. The 12 amino acids representing the bioactive peptide in A. thaliana have virtually been unchanged throughout the evolution of the angiosperms; however, the number of IDL and HSL genes varies between different orders and species. The phylogenetic analyses suggest that IDA, HSL2, and the related HSL1 gene, were present in the species that gave rise to the angiosperms. HAE has arisen from HSL1 after a genome duplication that took place after the monocot—eudicots split. HSL1 has also independently been duplicated in the monocots, while HSL2 has been lost in gingers (Zingiberales) and grasses (Poales). IDA has been duplicated in eudicots to give rise to functionally divergent IDL peptides. We postulate that the high number of IDL homologs present in the core eudicots is a result of multiple whole genome duplications (WGD). We substantiate the involvement of IDA and HAE/HSL2 homologs in abscission by providing gene expression data of different organ separation events from various species.