LC-MSn methods for saccharide characterization of monoglycosyl flavonoids using postcolumn manganese complexation
LC-MSn methods for saccharide characterization of monoglycosyl flavonoids using postcolumn manganese complexation
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DOI：
10.1021/ac048374o
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发表时间：
2005-03-15
影响因子：
7.4
通讯作者：
Brodbelt, JS
中科院分区：
文献类型：
--
作者：
Davis, BD;Brodbelt, JS
报道了一种简单的串联质谱方法，用于鉴别单糖基黄酮醇、黄酮和黄烷酮的异构体。[Mn（II）（L）（L-H）]（+）和[Mn（II）（L）（2）（L-H）]（+）物质的解离提供了独特的碎片离子，其允许将糖部分鉴定为葡萄糖、半乳糖、阿拉伯糖或木糖。类黄酮的糖基化位点也可以通过Mn络合物的断裂途径来确定。锰络合法适用于在线液相色谱-质谱分析，并测试了从富士苹果（苹果Borkh。CV.富士）和红洋葱（洋葱L.）。使用碎片数据从碰撞活化解离的去质子化的黄酮苷及其锰络合物，这些提取物中的主要黄酮类化合物进行了鉴定。
A simple tandem mass spectrometry method for differentiating isomeric monoglycosyl flavonols, flavones, and flavanones using manganese complexation is reported. Dissociation of the [Mn(II) (L) (L - H)](+) and [Mn(II) (L)(2) (L - H)](+) species provides unique fragment ions that allow the identification of the saccharide moiety as glucose, galactose, arabinose, or xylose. The glycosylation site of the flavonoid can also be determined by the fragmentation pathways of the Mn complexes. The Mn complexation method was adapted for on-line liquid chromatography-mass spectrometry analysis and tested using flavonoid extracts from Fuji apples (Malus domestica Borkh. cv. Fuji) and red onions (Allium cepa L.). Using fragmentation data obtained from collisional activated dissociation of the deprotonated flavonoid glycosides and their Mn complexes, the major flavonoid species in these extracts were identified.