The homeoprotein Hex is required for hemangioblast differentiation
The homeoprotein Hex is required for hemangioblast differentiation
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DOI：
10.1182/blood-2003-02-0634
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发表时间：
2003-10-01
期刊：
影响因子：
20.3
通讯作者：
Hromas, R
中科院分区：
文献类型：
--
作者：
Guo, Y;Chan, R;Hromas, R
第一代造血祖细胞和内皮祖细胞来源于称为成血管细胞的常见胚胎前体。调节成血管细胞分化为造血细胞和内皮细胞的遗传级联反应在很大程度上是未知的。一般来说，胚胎发育的大部分是由转录因子的时间和空间表达协调调节的，例如同源框（Hox）基因家族。我们和其他人分离了一个不同的同源异型盒基因，称为Hex（或Prh），它优先在造血和内皮细胞中表达。使用体外Hex（-/-）胚胎干（ES）细胞分化，在体内卵黄囊造血祖细胞测定，和嵌合小鼠分析，我们发现，Hex是所需的成血管细胞分化为最终的胚胎造血祖细胞，并在较小程度上内皮细胞。因此，Hex是成血管细胞分化为造血细胞和内皮细胞的一种新的调节剂。(C)2003年，美国血液学会。
The first hematopoietic and endothelial progenitors are derived from a common embryonic precursor termed the hemangioblast. The genetic cascades that regulate the differentiation of the hemangioblast to hematopoietic and endothelial cells are largely unknown. In general, much of embryonic development is coordinately regulated by temporal and spatial expression of transcription factors, such as the Homeobox (Hox) gene family. We and others isolated a divergent homeobox gene termed Hex (or Prh) that is preferentially expressed in hematopoietic and endothelial tells. Using in vitro Hex(-/-) embryonic stem (ES) cell differentiation, in vivo yolk sac hematopoietic progenitor assays, and chimeric mouse analysis, we found that Hex is required for differentiation of the hemangioblast to definitive embryonic hematopoietic progenitors and to a lesser extent endothelial cells. Therefore, Hex is a novel regulator of hemangioblast differentiation to hematopoietic and endothelial cells. (C) 2003 by The American Society of Hematology.