A tightly regulated molecular motor based upon T7 RNA polymerase
A tightly regulated molecular motor based upon T7 RNA polymerase
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DOI：
10.1021/nl0509125
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发表时间：
2005-09-01
期刊：
影响因子：
10.8
通讯作者：
McAllister, WT
中科院分区：
文献类型：
--
作者：
Pomerantz, RT;Ramjit, R;McAllister, WT
在纳米技术的许多应用中，材料或分子在纳米范围内的受控运动是必不可少的。在这里，我们报告了一种由DNA序列控制的改良形式的T7RNA聚合酶(RNAP)沿DNA捕获、移动和释放货物分子的过程。使用单分子方法，我们可视化了纳米设备的组装和操作，以及利用RNAP马达的旋转力和线性力的能力。
Controlled movement of materials or molecules within the nanometer range is essential in many applications of nanotechnology. Here we report the capture, movement, and release of cargo molecules along DNA by a modified form of T7 RNA polymerase (RNAP) in a manner that is controlled by the sequence of the DNA. Using single-molecule methods, we visualize the assembly and manipulation of nanodevices and the ability to harness rotary and linear forces of the RNAP motor.