T-CELL PROLIFERATION TO SUBINFECTIOUS SIV CORRELATES WITH LACK OF INFECTION AFTER CHALLENGE OF MACAQUES
T-CELL PROLIFERATION TO SUBINFECTIOUS SIV CORRELATES WITH LACK OF INFECTION AFTER CHALLENGE OF MACAQUES
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DOI：
10.1097/00002030-199410000-00004
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发表时间：
1994-10-01
期刊：
影响因子：
3.8
通讯作者：
BENVENISTE, RE
中科院分区：
文献类型：
--
作者：
CLERICI, M;CLARK, EA;BENVENISTE, RE
目的：分析猕猴暴露于SIV后产生保护作用的相关因素。方法：用不同稀释度的SIV接种猕猴外周血单个核细胞（PBMC），观察其对SIV包膜肽的体外增殖反应和SIV特异性抗体的产生。一些猕猴先前静脉注射暴露于亚传染性SIV剂量，随后在16个月后接受感染性直肠内SIV剂量的攻击。结果：暴露于感染性SIV剂量的猕猴的病毒特异性免疫反应的特征是产生抗体和弱或不可检测的t细胞介导的反应。相比之下，接种SIV剂量低于病毒血清转化和恢复所需阈值的猕猴对SIV包膜合成肽的反应表现出t细胞增殖。先前暴露于SIV的猕猴抵抗了随后的病毒攻击，而未暴露于SIV的幼稚猕猴全部被感染。结论：无法有效感染先前暴露于亚感染剂量SIV的猕猴表明，t细胞介导的反应可能具有长期的抗感染保护作用，艾滋病疫苗的设计应优化免疫反应的细胞部分。
Objectives: To analyze correlates of protection in macaques exposed to SIV.Methods: Peripheral blood mononuclear cells (PBMC) from macaques inoculated intrarectally with various dilutions of SIV were examined for their in vitro proliferative response to SIV envelope peptides and generation of SIV-specific antibodies. Some macaques previously exposed intravenously to subinfectious doses of SIV were subsequently challenged 16 months later with an infectious intrarectal dose of SIV.Results: The viral-specific immune responses of macaques exposed to infectious doses of SIV were characterized by generation of antibodies and weak or undetectable T-cell-mediated responses. In contrast, macaques inoculated with doses of SIV below the threshold required for seroconversion and recovery of virus exhibited T-cell proliferation in response to SIV envelope synthetic peptides. The macaques that had previously been exposed to SIV resisted the subsequent virus challenge, whereas the naive macaques (never exposed to SIV) all became infected.Conclusions: The inability to productively infect macaques previously exposed to subinfectious doses of SIV suggests that a T-cell-mediated response may confer long-term protection against infection, and that AIDS vaccines should be designed to optimize the cellular arm of the immune response.