ROLE OF MICROBIAL FLORA IN UREMIA .I. SURVIVAL TIMES OF GERMFREE LIMITED-FLORA AND CONVENTIONALIZED RATS AFTER BILATERAL NEPHRECTOMY AND FASTING
ROLE OF MICROBIAL FLORA IN UREMIA .I. SURVIVAL TIMES OF GERMFREE LIMITED-FLORA AND CONVENTIONALIZED RATS AFTER BILATERAL NEPHRECTOMY AND FASTING
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DOI：
10.1084/jem.123.2.239
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发表时间：
1966-01-01
影响因子：
15.3
通讯作者：
CARTER, D
中科院分区：
文献类型：
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作者：
EINHEBER, A;CARTER, D
采用无菌大鼠（GF）、有限菌群大鼠和常规大鼠（CONV）研究微生物菌群对一期双侧肾切除术后急性尿毒症存活时间的影响。在切除肾前，GF大鼠分别被单一污染（白色单胞菌）、双重污染（白色单胞菌和奇异单胞菌）、四污染（白色单胞菌、奇异单胞菌、粪单胞菌和大肠杆菌）或“常规”污染（暴露于普通大鼠盲肠内容物中含有的混合微生物菌群）。在切除肾脏和剥夺食物和水（无尿死亡）后：“a)除一只外，所有GF大鼠在3个实验中都比CONV大鼠活得长；21只GF大鼠（127小时）比24只CONV大鼠（75小时）平均多活2天；没有性别差异；b)随着菌群复杂性的增加，菌群数量有限的大鼠相应地死亡时间越早；四价污染大鼠的生存时间明显短于GF大鼠，与CONV大鼠的生存时间无统计学差异。假性肾切除术和剥夺食物和水（延迟饥饿死亡）后：a)所有大鼠，不论其微生物状态或性别，都比禁食切除肾的同伴活得长。CONV组大鼠比无尿组长约2.5周，GF组大鼠长约1周；b)所有雄性和雌性CONV大鼠的寿命都比GF大鼠长约1周；c)无论微生物状态如何，所有雄性的存活时间都比雌性长；平均相差4天。对无尿或饥饿耐受性的差异与初始体重或体重减轻率无关。因此，大鼠的微生物状态显著影响其忍受急性无尿和食物和水剥夺的致命后遗症的能力。
Germfree (GF), limited-flora, and conventionalized (CONV) rats were used to study the effects of the microbial flora on survival time after acute uremia produced by a one-stage bilateral nephrectomy. GF rats, in advance of nephrectomy, were either monocontaminated (S. albus), dicontaminated (S. albus and P. mirabilis), tetracontaminated (S. albus, P. mirabilis, S. faecalis, and E. coli), or "conventionalized" "(exposed to the mixed microbial flora contained in the cecal contents of ordianry rats). After nephrectomy and food and water deprivation (death from anuria): "a) All GF rats but one outlived their CONV counterparts in each of the 3 experiments; the 21 GF rats (127 hr) lived, on the average, 2 days longer than did the 24 CONV rats (75 hr); no sex difference was demonstrated; b) The rats with a limited flora died correspondingly sooner as the complexity of their flora increased; survival time of the tetra-contaminated rats was significantly shorter than that of the GF rats, and statistically no different from that of the CONV rats. After sham nephrectomy and food and water deprivation (delayed death from starvation): a) All rats, irrespective of microbial status or sex, outlived their fasting nephrectomized partners. The CONV rats endured starvation approximately 2.5 wk longer than they did anuria, and the GF rats 1 wk longer; b) All CONV rats, both male and female, outlived their respective GF counterparts by about 1 wk; c) All males, irrespective of microbial status, survived longer than did the females; the average difference was 4 days. The differences in tolerance to anuria or starvation did not correlate with initial body weight or rate of weight loss. Thus, the microbial status of the rat significantly influences its ability to endure the lethal sequelae of acute anuria and of food and water deprivation.