Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers
Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers
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DOI：
10.1016/j.mce.2006.09.014
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发表时间：
2007-01-15
影响因子：
4.1
通讯作者：
Gui, Han-Fang
中科院分区：
文献类型：
--
作者：
Xia, Wei;Zhou, Li;Gui, Han-Fang
DMRT 1在不同脊椎动物中的表达模式不同，因此被认为在性别决定和性腺分化中发挥着不同的作用。石斑鱼的性腺先发育为卵巢，再发育为精巢，是研究性别分化和决定的理想模型。本研究克隆了斜带石斑鱼（Epineoderus coioides）DMRT 1基因的全长cDNA，并制备了相应的抗EcDMRT 1的抗血清，以研究DMRT 1与性逆转的关系。一个重要的发现是，石斑鱼DMRT]不仅在不同阶段的性腺中差异表达，而且还限于精子发生的特定阶段和特定细胞。石斑鱼DMRT 1蛋白仅存在于精原细胞、初级精母细胞和次级精母细胞中，而支持细胞中不存在DMRT 1蛋白。EcSox 3不仅在卵原细胞和不同发育阶段的卵母细胞中表达，而且在支持细胞和精原细胞中也有表达，而EcSox 9仅在支持细胞中表达。这些结果表明，DMRT 1基因可能是一个更特异的精子发生性别分化基因，在精原细胞到精母细胞的特定阶段发挥作用。此外，在石斑鱼DMRT 1中没有发现内含子，也没有检测到重复的DMRT 1基因。这一发现表明，能够进行快速转录周转的无内含子DMRT 1可能是一个重要的基因，刺激精子发生的雌雄同体性腺。(c)2006年由Elsevier爱尔兰有限公司出版。
DMRT1 has been suggested to play different roles in sex determination and gonad differentiation, because different expression patterns have been reported among different vertebrates. The groupers, since their gonads first develop as ovary and then reverse into testis, have been thought as good models to study sex differentiation and determination. In this study, we cloned the full-length cDNAs of DMRT] gene from orange-spotted grouper (Epinephelus coioides), and prepared corresponding anti-EcDMRT1] antiserum to study the relationship of DMRT] to sex reversal. One important finding is that the grouper DMRT] is not only differentially expressed in different stage gonads, but also restricted to specific stages and specific cells of spermatogenesis. Grouper DMRT1 protein exists only in spermatogonia, primary spermatocytes and secondary spermatocytes, but not in the supporting Sertoli cells. Moreover, we confirmed that EcSox3 is expressed not only in oogonia and different stage oocytes, but also in Sertoli cells and spermatogonia, and EcSox9 is expressed only in Sertoli cells. The data suggested that grouper DMRT1 might be a more specific sex differentiation gene for spermatogenesis, and play its role at the specific stages from spermatogonia to spermatocytes. In addition, no introns were found in the grouper DMRT1, and no duplicated DMRT1, genes were detected. The finding implicates that the intronless DMRT1 that is able to undergo rapid transcriptional turnover might be a significant gene for stimulating spermatogenesis in the protogynous hermaphroditic gonad. (c) 2006 Published by Elsevier Ireland Ltd.