TRANSFORMATION OF AN INTERLEUKIN-3-DEPENDENT HEMATOPOIETIC-CELL LINE BY THE CHRONIC MYELOGENOUS LEUKEMIA-SPECIFIC P210BER/ABL PROTEIN
TRANSFORMATION OF AN INTERLEUKIN-3-DEPENDENT HEMATOPOIETIC-CELL LINE BY THE CHRONIC MYELOGENOUS LEUKEMIA-SPECIFIC P210BER/ABL PROTEIN
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DOI：
10.1073/pnas.85.23.9312
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发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
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作者：
DALEY, GQ;BALTIMORE, D
P210bcr/abl蛋白几乎与所有人类慢性髓性白血病病例有关。与Abelson小鼠白血病病毒相关的P160gag/v-abl癌基因产物不同，P210bcr/abl不会转化NIH 3T3成纤维细胞。为了评估P210bcr/abl是否可能转化造血细胞类型，使用编码P210bcr/abl的逆转录病毒构建物感染骨髓源性白细胞介素3依赖性Ba/F3细胞系。对于P160gag/v-abl，表达P210bcr/abl的细胞系不依赖于生长因子，在裸鼠中具有致瘤性。没有证据表明不依赖因子的细胞系产生自分泌的白细胞介素3。这些实验表明P210bcr/abl能够将造血细胞类型转化为致瘤性。
The P210bcr/abl protein is associated with virtually every case of human chronic myelogenous leukemia. Unlike the related P160gag/v-abl oncogene product of Abelson murine leukemia virus, P210bcr/abl does not transform NIH 3T3 fibroblasts. To assess whether P210bcr/abl might transform hematopoietic cell types, retroviral constructs encoding P210bcr/abl were used to infect the bone marrow-derived interleukin 3-dependent Ba/F3 cell line. As for P160gag/v-abl, cell lines expressing P210bcr/abl were growth factor independent and tumorigenic in nude mice. No evidence for autocrine production of interleukin 3 by factor-independent cell lines was found. These experiments establish that P210bcr/abl can transform hematopoietic cell types to tumorigenicity.