Transforming growth factor-, interleukin-17, and IL-23 gene expression profiles associated with human peri-implantitis
Transforming growth factor-, interleukin-17, and IL-23 gene expression profiles associated with human peri-implantitis
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DOI：
10.1111/clr.12846
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发表时间：
2017-07-01
影响因子：
4.3
通讯作者：
Bastos, Marta Ferreira
中科院分区：
文献类型：
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作者：
Mardegan, Gustavo Pereira;Shibli, Jamil Awad;Bastos, Marta Ferreira
目的研究IL-23/Th 17和Treg相关细胞因子TGF-β在种植体周围炎中的mRNA表达。本研究描述了健康和患病种植体周围组织中IL-17、IL-23和TGF-β基因的表达水平，并将这些数据与放射学骨丢失相关联。（20名健康对照，平均年龄为59.4 ± 6.3岁，20名种植体周围炎患者，平均年龄为56.6 ± 5.5岁）入组本研究，采用实时荧光定量PCR（RT-PCR）检测IL-17、IL-23和TGF-β 1基因的表达。结果在健康对照组的活检组织中观察到较高水平的TGF-β 1 mRNA，种植体周围炎组IL-23 mRNA水平明显升高结论种植体周围炎患者外周血IL-23水平升高，TGF-β水平降低，Th 17细胞应答占优势。
ObjectivesThe mRNA expression profiles of IL-23/Th17 and the Treg-associated cytokine TGF- in peri-implantitis are currently under research. This study characterized the IL-17, IL-23, and TGF- gene expression levels in healthy and diseased peri-implant tissues and correlated these data with radiographic bone loss.Material and MethodsPeri-implant soft tissues from 40 subjects (20 healthy controls with mean age of 59.46.3, and 20 with peri-implantitis with mean age of 56.65.5) were enrolled in this study, and real-time PCR (RT-PCR) was used to define the profile of IL-17, IL-23, and TGF- gene expression.ResultsHigher levels of TGF- mRNA were observed in biopsies taken from healthy controls, and the IL-23 mRNA levels were significantly increased in the peri-implantitis group (P0.05).ConclusionsData presented in this report demonstrated a predominant Th17 response in peri-implantitis subjects based on the higher levels of IL-23 and lower levels of TGF- detected.